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Electrophysiological Characterization of Membrane Transport Proteins
Annual Review of Biophysics, 2013Active transport in biological membranes has been traditionally studied using a variety of biochemical and biophysical techniques, including electrophysiology. This review focuses on aspects of electrophysiological methods that make them particularly suited for the investigation of transporter function.
Grewer, C. +3 more
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1974
The relative constancy of the internal environment of the cell is maintained by a variety of transport systems which are located within the membrane. These transport systems serve to regulate the entrance and exit of various solutes concerned with the metabolic activity of the cell.
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The relative constancy of the internal environment of the cell is maintained by a variety of transport systems which are located within the membrane. These transport systems serve to regulate the entrance and exit of various solutes concerned with the metabolic activity of the cell.
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Biosensors based on membrane transport proteins
Biosensors and Bioelectronics, 1991We propose a novel class of biosensors based on membrane bound receptors or transport proteins as the sensing element. The protein is incorporated in a planar lipid bilayer which covers the transducer. The transducer may detect an electric current, a voltage, or a change in fluorescence.
H, Kiefer +4 more
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Protein transport across the ER membrane
Trends in Biochemical Sciences, 1990Protein transport across the endoplasmic reticulum (ER) membrane may be divided into two phases: an initiation or targeting cycle, which has been fairly well characterized, and the actual transfer of the polypeptide chain through the membrane, the mechanism of which is still unknown.
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Determining Protein Transport to the Plasma Membrane
Current Protocols in Cell Biology, 2000AbstractMany integral membrane proteins synthesized in the endoplasmic reticulum ultimately arrive at the cell surface to contact the cell environment. During transit through the Golgi and trans‐Golgi network, proteins acquire post‐translational modifications that can be used to track the appearance of such modified proteins at the cell surface ...
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Bacterial Membrane Transport Proteins
1976Bacterial transport systems are historically associated with the acceptance of the idea that the crossing of the cell membrane by a physiologically significant solute was mediated by the specialized operation of a protein or an array of molecules including specific proteins.
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Binding proteins and membrane transport.
Annals of the New York Academy of Sciences, 1976The recent studies have clearly established two types of active transport systems. One type is membrane-bound and can be observed in membrane vesicles and the other type is osmotic-shock-sensitive and requires binding proteins to produce active transport.
D L, Oxender, S, Quay
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Protein Transport Across Membranes
2015Mitochondria contain their own DNA, they divide throughout interphase and are distributed randomly between daughter cells during cell division. Most mitochondrial proteins are encoded in the nucleus and produced in the cytosol. They are then imported into the mitochondria via the TOM/TIM transport system, which recognises them by a specific ...
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De novo design of membrane transport proteins
Proteins: Structure, Function and Bioinformatics, 2022Peilong Lu
exaly