Results 221 to 230 of about 281,251 (291)

Profiling Protein-Peptide Interactions by Yeast Surface Display. [PDF]

Curr Protoc
Hurley JD, Kruse AC.
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Research on Capacitated Multi-Ship Replenishment Path Planning Problem Based on the Synergistic Hybrid Optimization Algorithm. [PDF]

Biomimetics (Basel)
Yang L, Chen Q, Mu J, Liu T, Li X, Cai S.   +5 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

scDynaBar: A Step-By-Step Experimental and Computational Guide for Time-Resolved CRISPR Barcoding at Single-Cell Resolution. [PDF]

Bio Protoc
Andres-Lopez Y, El Khouri-Gonzalez C, Hernando-Herraez I.   +2 more
europepmc   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

An Improved Red-Billed Blue Magpie Optimization for Function Optimization and Engineering Problems. [PDF]

Biomimetics (Basel)
Han C, Zhang T, Han H, Dai W, Wu W.
europepmc   +1 more source

Protocol for processing and analyzing multiplexed images improves lymphatic cell identification and spatial architecture in human tissue. [PDF]

STAR Protoc
Smith JA, Bloss DT, Williams MD, Posgai AL, Brusko TM, Atkinson MA, Wasserfall CH, Brusko MA.   +7 more
europepmc   +1 more source

A Dual-Camera Edge Sensing Framework with Zone-Aware Multi-Object Tracking for Sensorless Smart Vending Cabinets. [PDF]

Sensors (Basel)
Buriboev AS, Rajabov F, Buriboev S, Allanyazov R, Sharipov G, Abduvaytov A, Akhmedova A, Sobirov R, Shin SM, Lee C, Jeon HS.   +10 more
europepmc   +1 more source

Micro-phenomenology of immersion and perceived presences under DMT. [PDF]

Neurosci Conscious
Sanders JW, Millière R, Demšar E, Daily ZG, Erritzoe D, Carhart-Harris R, Timmermann C.   +6 more
europepmc   +1 more source