Results 331 to 340 of about 1,613,624 (375)

Evidence that immune RNA is messenger RNA

Cellular Immunology, 1976
Abstract Exposure of rabbit spleen cell cultures to i-RNA isolated from T2 phage-exposed rabbit peritoneal exudate cells induces the synthesis of antigen and allotype specific 19S proteins even in the presence of actinomycin D. The same i-RNA directs the synthesis of proteins with comparable properties in cell-free extracts prepared from mouse L ...
P, Bilello, M, Fishman, G, Koch
openaire   +2 more sources

Intranuclear Trafficking of Messenger RNA

Critical Reviews™ in Eukaryotic Gene Expression, 1999
Within the nucleus, protein-encoding genes are transcribed into messenger RNA by RNA polymerase II. Messenger RNAs migrate to the cytoplasm, but before reaching their final destination the primary transcripts must undergo a series of modifications that include 5'-capping, splicing, and 3'-cleavage/polyadenylation.
M, Carmo-Fonseca, N, Custódio, A, Calado   +2 more
openaire   +2 more sources

A CRISPR cut for messenger RNAs

Lab Animal, 2020
To achieve knockdown rather than knockout of particular genes, a new paper demonstrates a CRISPR/Cas13 method that can efficiently edit mRNA in zebrafish, medaka, killifish, and mouse embryos.
Rebecca, Leech, Karuna, Sampath
openaire   +2 more sources

Monocistronic messenger RNA in yeast

Journal of Molecular Biology, 1973
Abstract We have determined the rate of polypeptide chain synthesis on different size polysomes in yeast. The completion time for the average polypeptide chain in vivo at 23 °C is two minutes by this technique and is in good agreement with values we have determined by other independent methods.
N S, Petersen, C S, McLaughlin
openaire   +2 more sources

RNA decay by messenger RNA interferases.

Methods in enzymology, 2008
Two abundant toxin-antitoxin (TA) gene families, relBE and mazEF, encode mRNA cleaving enzymes whose ectopic overexpression abruptly inhibits translation and thereby induces a bacteriostatic condition. Here we describe and discuss protocols for the overproduction, purification, and analysis of mRNA cleaving enzymes such as RelE of Escherichia coli and ...
Christensen-Dalsgaard M, Overgaard M, Winther KS, Gerdes K   +3 more
openaire   +4 more sources

Radiation sensitivity of messenger RNA

Molecular and General Genetics MGG, 1979
Messenger RNA function is inactivated by irradiation with ultraviolet light. A unit length mRNA (in bases) is 2-3 times more sensitive than a unit length of DNA (in base pairs) with respect to the inactivation of template function. These data stem from four experimental systems all of which do not repair DNA: the translation of E.
Ponta, H., Pfennig-Yeh, M. L., Wagner, E. F., Schweiger, M., Herrlich, P.   +4 more
openaire   +2 more sources

The structure of pre-messenger RNA and messenger RNA from erythroid cells

1977
Pre-mRNA fractions (greater than 45 S) were characterized by electron microscopy. High salt concentrations (0.2 M ammonium acetate, pH 8) yield linear molecules of different length (0.5--17 micrometer). In 10% of the molecules a compact-nonlinear contour (cn-contour) is detectable at one end.
A M, Ladhoff, B J, Thiele, C, Coutelle, S, Rosenthal   +3 more
openaire   +2 more sources

The Decay of Bacterial Messenger RNA

1996
Publisher Summary The many demonstrations that the Escherichia coli (E. coli ) rne gene product (RNase E) is involved in messenger RNA (mRNA) decay have given real impetus to the study of this unusual protein's properties and role. The recent attention given to the polyadenylylation of bacterial mRNAs and the discovery that polyadenylylation ...
D P, Nierlich, G J, Murakawa
openaire   +2 more sources

The instability of T4 messenger RNA

Journal of Molecular Biology, 1967
We have studied the stability of messenger RNA in T4-infected cells of Escherichia coli CR34 that had been sensitized to actinomycin by treatment with EDTA. T4 infection is shown to proceed normally in these EDTA-treated cells. Pulse-labeled messenger RNA both early and late in infection decays with a half-life of 3·5 minutes at 37°C.
R, Greene, D, Korn
openaire   +2 more sources

Hormone inducible messenger RNA

Life Sciences, 1975
Abstract Steroid hormones regulate the synthesis of specific proteins in target tissue by controlling the synthesis of the corresponding messenger RNA's. In the chick oviduct, estrogen and progesterone induce the synthesis and accumulation of the specific messenger RNA's for the hormone-induced egg-white proteins, ovalbumin and avidin, respectively ...
S L, Woo, B W, O'Malley
openaire   +2 more sources