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Expert Opinion on Biological Therapy, 2004
RNA is the only molecule known to recapitulate all biochemical functions of life: definition, control and transmission of genetic information, creation of defined three-dimensional structures, enzymatic activities and storage of energy. Because of its versatility and thanks to several recent scientific breakthroughs, RNA became the focus of intense ...
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RNA is the only molecule known to recapitulate all biochemical functions of life: definition, control and transmission of genetic information, creation of defined three-dimensional structures, enzymatic activities and storage of energy. Because of its versatility and thanks to several recent scientific breakthroughs, RNA became the focus of intense ...
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RNA decay by messenger RNA interferases.
Methods in enzymology, 2008Two abundant toxin-antitoxin (TA) gene families, relBE and mazEF, encode mRNA cleaving enzymes whose ectopic overexpression abruptly inhibits translation and thereby induces a bacteriostatic condition. Here we describe and discuss protocols for the overproduction, purification, and analysis of mRNA cleaving enzymes such as RelE of Escherichia coli and ...
Christensen-Dalsgaard M +3 more
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Vaccination With Messenger RNA
2006As an alternative to DNA-based vaccines, messenger RNA (mRNA)-based vaccines present additional safety features: no persistence, no integration in the genome, no induction of autoantibodies. Moreover, mRNA which are generated by in vitro transcription, are easy to produce in large amounts and very high purity.
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2015
This chapter is focused on the localization of mRNAs1-6, including the localization of microRNAs in neurons. It describes fluorescence in situ hybridization (FISH). Fluorescence uses in situ hybridization wherein a complementary antisense nucleic acid probe is labelled typically with a fluorescent probe.
David Elliott, Michael Ladomery
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This chapter is focused on the localization of mRNAs1-6, including the localization of microRNAs in neurons. It describes fluorescence in situ hybridization (FISH). Fluorescence uses in situ hybridization wherein a complementary antisense nucleic acid probe is labelled typically with a fluorescent probe.
David Elliott, Michael Ladomery
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Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction.
Science, 1992Peng Liang, A. Pardee
semanticscholar +1 more source
2011
Twenty years after the seminal observation of Wolff et al. that injection of naked RNA and DNA vectors results in protein expression in vivo, messenger RNA (mRNA) vaccines have found entry into clinical development. Through improved vector design, formulation, and delivery, mRNA, initially perceived as unstable and difficult to manipulate, has been ...
Jochen Probst +5 more
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Twenty years after the seminal observation of Wolff et al. that injection of naked RNA and DNA vectors results in protein expression in vivo, messenger RNA (mRNA) vaccines have found entry into clinical development. Through improved vector design, formulation, and delivery, mRNA, initially perceived as unstable and difficult to manipulate, has been ...
Jochen Probst +5 more
openaire +1 more source
Single-cell messenger RNA sequencing reveals rare intestinal cell types
Nature, 2015Dominic Grün +7 more
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