Results 111 to 120 of about 40,506 (282)

ranacapa: An R package and Shiny web app to explore environmental DNA data with exploratory statistics and interactive visualizations. [PDF]

, 2018
Environmental DNA (eDNA) metabarcoding is becoming a core tool in ecology and conservation biology, and is being used in a growing number of education, biodiversity monitoring, and public outreach programs in which professional research scientists engage
Cowen, Madeline C, Curd, Emily E, Freise, Amanda C, Gold, Zachary J, Kandlikar, Gaurav S, Kraft, Nathan JB, Kushner, David J, Meyer, Rachel S, Moberg-Parker, Jordan, Sprague, Joshua   +9 more
core  

Quantitative monitoring of diverse fish communities on a large scale combining eDNA metabarcoding and qPCR

Molecular Ecology Resources, 2022
Environmental DNA (eDNA) metabarcoding is an effective method for studying fish communities but allows only an estimation of relative species abundance (density/biomass).
D. Pont, P. Meulenbroek, Vincenz Bammer, Tony Dejean, T. Erős, Pauline Jean, M. Lenhardt, Christoffer Nagel, L. Pekárik, M. Schabuss, B. Stoeckle, E. Stoica, H. Zornig, A. Weigand, A. Valentini   +14 more
semanticscholar   +1 more source

How quantitative is metabarcoding: A meta‐analytical approach [PDF]

Molecular Ecology, 2018
AbstractMetabarcoding has been used in a range of ecological applications such as taxonomic assignment, dietary analysis and the analysis of environmental DNA. However, after a decade of use in these applications there is little consensus on the extent to which proportions of reads generated corresponds to the original proportions of species in a ...
John K. Pinnegar, John K. Pinnegar, Philip D. Lamb, Ewan Hunter, Ewan Hunter, Martin I. Taylor, Simon Creer, Richard G. Davies   +7 more
openaire   +4 more sources

Plankton communities today and tomorrow—potential impacts of multiple global change drivers and marine heatwaves

Limnology and Oceanography, EarlyView.
Abstract In the context of global change, marine organisms are subjected not only to gradual changes in abiotic parameters, but also to an increasing number of extreme events, such as heatwaves. However, we still know little about the influence of heatwaves on the structure of marine communities, and experimental studies are needed to test the impact ...
Cédric L. Meunier, Josefin Schmidt, Antonia Ahme, Areti Balkoni, Katharina Berg, Lea Blum, Maarten Boersma, Jan D. Brüwer, Bernhard M. Fuchs, Luis Gimenez, Maïté Guignard, Ruben Schulte‐Hillen, Bernd Krock, Johannes Rick, Herwig Stibor, Maria Stockenreiter, Simon Tulatz, Felix Weber, Antje Wichels, Karen Helen Wiltshire, Sylke Wohlrab, Inga V. Kirstein   +21 more
wiley   +1 more source

Molecular metrics to monitor ecological status of large rivers: Implementation of diatom DNA metabarcoding in the Joint Danube Survey 4

Ecological Indicators
The Joint Danube Survey (JDS) is a regular transnational survey to monitor the quality of the Danube and its main tributaries, in accordance with the EU Water Framework Directive.
Kálmán Tapolczai, Teofana Chonova, Dana Fidlerová, Jarmila Makovinská, Demetrio Mora, Alexander Weigand, Jonas Zimmermann   +6 more
doaj   +1 more source

Development of a multigenic metabarcoding of prokaryotic microbiota: application to Bacteria and Archaea of agronomical interest encountered in the rice rhizosphere of Camargue [PDF]

, 2017
It is our hypothesis that soil and plant microbiota are playing an eminent role in shaping both natural ecosystems and agricultural production systems. It is now widely admitted that bacterial communities show a great diversity in the rhizosphere and in ...
Blondin, Laurence, Ogier, Jean-Claude, Pieretti, Isabelle, Roumagnac, Philippe   +3 more
core  

It’s raining species: Rainwash eDNA metabarcoding as a minimally invasive method to assess tree canopy invertebrate diversity

bioRxiv, 2022
Forest canopies are a highly diverse ecosystem, but despite several decades of intense research, there remain substantial gaps in our knowledge of their biodiversity and ecological interactions. One fundamental challenge in canopy research is the limited
Till-Hendrik Macher, Robin Schütz, Thomas Hörren, A. Beermann, F. Leese   +4 more
semanticscholar   +1 more source

Observation Bias in Metabarcoding

Molecular Ecology Resources
ABSTRACTDNA metabarcoding is subject to observation bias associated with PCR and sequencing, which can result in observed read proportions differing from actual species proportions in the DNA extract. Here, we amplify and sequence a mock community of known composition containing marine fishes and cetaceans using four different primer sets and a variety
Megan R. Shaffer, Elizabeth Andruszkiewicz Allan, Amy M. Van Cise, Kim M. Parsons, Andrew Olaf Shelton, Ryan P. Kelly   +5 more
openaire   +2 more sources

DNA Metabarcoding as a Tool for Disentangling Food Webs in Agroecosystems [PDF]

Insects, 2020
Better knowledge of food webs and related ecological processes is fundamental to understanding the functional role of biodiversity in ecosystems. This is particularly true for pest regulation by natural enemies in agroecosystems. However, it is generally difficult to decipher the impact of predators, as they often leave no direct evidence of their ...
Thierry Brévault, Laure Benoit, Maxime Galan, Maxime Galan, Julien Haran, Ahmadou Sow   +5 more
openaire   +6 more sources

Validating environmental DNA metabarcoding for marine fishes in diverse ecosystems using a public aquarium

Environmental DNA, 2020
Environmental DNA metabarcoding has been widely touted as a powerful tool for monitoring biodiversity in marine ecosystems. However, this method still requires thorough validation and standardization before it can be widely applied for ecological ...
Kevin C. Morey, Timothy J. Bartley, Robert H. Hanner   +2 more
doaj   +1 more source