Results 111 to 120 of about 30,204 (224)
Methods in Ecology and Evolution, EarlyView.Abstract Long‐read amplicon sequencing is now routine for fungal metabarcoding, and locating the internal transcribed spacer (ITS) subregions within reads has become a throughput bottleneck. Existing extractors were designed for shorter reads and for datasets in which near‐identical sequences are common, and neither assumption holds for Oxford Nanopore
Aaron O'Brien +4 more
wiley +1 more source
Enhanced fish detection sensitivity in aquatic systems using environmental RNA
Methods in Ecology and Evolution, EarlyView.Abstract Environmental RNA (eRNA) can potentially augment the biological monitoring ability of the established environmental DNA (eDNA) application in conservation. Despite faster degradation, environmental RNA could offer unique advantages: it may help reduce the legacy signal compared to eDNA, provide insights into physiological status through gene ...
Ana Ramón‐Laca +9 more
wiley +1 more source
Comparison of GoFish and metabarcoding.
, 2018 A. Number of detections by method for the 11 species and 34 samples shown in Fig 3. A detection refers to a positive GoFish or metabarcoding result for a single species in a single sample, which corresponds to one cell in Fig 3 grid.
Mark Y. Stoeckle (139976) +2 more
core +1 more source
Methods in Ecology and Evolution, EarlyView.Abstract As long‐read amplicon sequencing becomes routine for fungal metabarcoding, species‐level abundance estimation from ITS amplicons remains limited by naive best‐hit classification. Assigning each read to its single highest‐scoring reference misattributes reads among closely related species that share similar ITS sequences, and divides the reads ...
Aaron O'Brien +4 more
wiley +1 more source
The Euphrates-Tigris catchment area is one of the major drainage basins in the Middle East but climate change, overuse of waters and subsequent salinization has brought this region to the brink of ecological devastation.
Al-Shaheen,Maitham +17 more
core +1 more source
Animal Conservation, EarlyView.Using fecal DNA metabarcoding, we quantified dietary overlap among reintroduced fishers and sympatric coyotes, bobcats, and Pacific martens in Washington's North Cascades. Niche overlap was substantial for common prey but varied with body size, revealing fine‐scale resource partitioning that may limit fishers' access to energetically efficient prey ...
Kayla A. Shively +7 more
wiley +1 more source
Animal Conservation, EarlyView.Monitoring semi‐aquatic mammals is essential for their conservation, but it is made difficult by their elusive lifestyle and generally low abundance. Eurasian otter (Lutra lutra) monitoring has traditionally relied on scat surveys, but eDNA and camera‐trapping are emerging as promising alternatives.
Simon Lacombe +15 more
wiley +1 more source
Assessing the host plants of the invasive apricot aphid, Myzus mumecola (Hemiptera: Aphididae)
Agricultural and Forest Entomology, EarlyView.We investigated the host plants of the invasive apricot aphid Myzus mumecola (Hemiptera: Aphididae), an emerging pest species in Europe, by combining molecular and ecological approaches. The winter host plant was confirmed as apricot by both methods.
Marta Chignola +2 more
wiley +1 more source
The dual crisis: Climate change simultaneously drives pollinator decline and pest outbreaks
Agricultural and Forest Entomology, EarlyView.Climate change acts as an asymmetric ecological filter, favouring r‐selected pest traits (rapid reproduction, generalism) while disadvantaging K‐selected pollinators. For every 1°C of warming, bee species richness declines ~25% since the 1990s, while pest‐induced crop losses increase by 10%–25%. A network‐centric approach integrating climate‐responsive
Diriba Fufa Serdo
wiley +1 more source
Acta Ophthalmologica, EarlyView.Abstract Purpose Infectious keratitis (IK) is a vision‐threatening condition with diverse microbial aetiologies, yet conventional culture‐based diagnostics often have limited sensitivity. We investigated whether 16S/18S rRNA amplicon‐based next‐generation sequencing (NGS) can enhance aetiological assessment when used alongside standard microbiological ...
Sandra M. Skovdal +6 more
wiley +1 more source