Isotope-Assisted Metabolic Flux Analysis: A Powerful Technique to Gain New Insights into the Human Metabolome in Health and Disease [PDF]
Metabolites, 2022 Cell metabolism represents the coordinated changes in genes, proteins, and metabolites that occur in health and disease. The metabolic fluxome, which includes both intracellular and extracellular metabolic reaction rates (fluxes), therefore provides a ...
Bilal Moiz +4 more
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Systematic comparison of local approaches for isotopically nonstationary metabolic flux analysis [PDF]
Frontiers in Plant Science, 2023 Quantification of reaction fluxes of metabolic networks can help us understand how the integration of different metabolic pathways determine cellular functions.
Sebastian Huß +3 more
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Micro-Slab Coil Design for Hyperpolarized Metabolic Flux Analysis in Multiple Samples [PDF]
Bioengineering, 2022 Abnormal metabolism is a hallmark of cancer cells. Accumulating evidence suggests that metabolic changes are likely to occur before other cellular responses in cancer cells upon drug treatment.
Geonhui Lee +7 more
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13C-metabolic flux analysis reveals metabolic rewiring in HL-60 neutrophil-like cells through differentiation and immune stimulation [PDF]
Metabolic Engineering CommunicationsNeutrophils are innate immune cells and the first line of defense for the maintenance of homeostasis. However, our knowledge of the metabolic rewiring associated with their differentiation and immune stimulation is limited.
Takeo Taniguchi +2 more
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Metabolic Flux Analysis—Linking Isotope Labeling and Metabolic Fluxes [PDF]
Metabolites, 2020 Metabolic flux analysis (MFA) is an increasingly important tool to study metabolism quantitatively. Unlike the concentrations of metabolites, the fluxes, which are the rates at which intracellular metabolites interconvert, are not directly measurable ...
Yujue Wang +4 more
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Robustifying Experimental Tracer Design for13C-Metabolic Flux Analysis [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 13C metabolic flux analysis (MFA) has become an indispensable tool to measure metabolic reaction rates (fluxes) in living organisms, having an increasingly diverse range of applications.
Martin Beyß +4 more
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NetFlow: A tool for isolating carbon flows in genome-scale metabolic networks
Metabolic Engineering Communications, 2021 1. Abstract: Genome-scale stoichiometric models (GSMs) have been widely utilized to predict and understand cellular metabolism. GSMs and the flux predictions resulting from them have proven indispensable to fields ranging from metabolic engineering to ...
Sean G. Mack, Ganesh Sriram
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iScience, 2022 Summary: Glucose homeostasis is maintained by modulation of metabolic flux. Enzymes and metabolites regulate the involved metabolic pathways. Dysregulation of glucose homeostasis is a pathological event in obesity.
Saori Uematsu +13 more
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iScience, 2020 Summary: Cellular metabolism is dynamic, but quantifying non-steady metabolic fluxes by stable isotope tracers presents unique computational challenges.
Lake-Ee Quek +15 more
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Metabolic traits specific for lipid-overproducing strain of Mucor circinelloides WJ11 identified by genome-scale modeling approach [PDF]
PeerJ, 2019 The genome-scale metabolic model of a lipid-overproducing strain of Mucor circinelloides WJ11 was developed. The model (iNI1159) contained 1,159 genes, 648 EC numbers, 1,537 metabolites, and 1,355 metabolic reactions, which were localized in different ...
Nattapat Isarankura Na Ayudhya +4 more
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