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Metabolic Flux Analysis for Escherichia coli by Flux Balance Analysis
2014Conventional metabolic flux analysis (MFA) of Escherichia coli wild type and of pathway gene knockout mutants cultivated under anaerobic condition is explained in detail in this chapter. To place the MFA results into the context of the literature, the regulation of central carbon metabolism in terms of catabolite regulation by the phosphotransferase ...
Yu, Matsuoka, Kazuyuki, Shimizu
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Flux Balance Analysis of Photoautotrophic Metabolism
Biotechnology Progress, 2005AbstractPhotosynthesis is the principal process responsible for fixation of inorganic carbon dioxide into organic molecules with sunlight as the energy source. Potentially, many chemicals could be inexpensively produced by photosynthetic organisms.
Avantika A, Shastri, John A, Morgan
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High-resolution 13C metabolic flux analysis
Nature Protocols, 2019Precise quantification of metabolic pathway fluxes in biological systems is of major importance in guiding efforts in metabolic engineering, biotechnology, microbiology, human health, and cell culture. 13C metabolic flux analysis (13C-MFA) is the predominant technique used for determining intracellular fluxes.
Christopher P. Long +1 more
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1997
Abstract Flux analysis is a method of organizing data which shows the quantitative relationships among all the metabolic events going on in a fermentation. It is a simple description of what is actually happening in the fermentation and does not, in itself, consider the theory of how fermentations are limited or controlled.
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Abstract Flux analysis is a method of organizing data which shows the quantitative relationships among all the metabolic events going on in a fermentation. It is a simple description of what is actually happening in the fermentation and does not, in itself, consider the theory of how fermentations are limited or controlled.
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13C flux analysis of cyanobacterial metabolism
Photosynthesis Research, 2014(13)C metabolic flux analysis (MFA) has made important contributions to our understanding of the physiology of model strains of E. coli and yeast, and it has been widely used to guide metabolic engineering efforts in these microorganisms. Recent advancements in (13)C MFA methodology combined with publicly available software tools are creating new ...
Adeola O, Adebiyi +2 more
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Isotopically Nonstationary 13C Metabolic Flux Analysis
2013(13)C metabolic flux analysis (MFA) is a powerful approach for quantifying cell physiology based upon a combination of extracellular flux measurements and intracellular isotope labeling measurements. In this chapter, we present the method of isotopically nonstationary (13)C MFA (INST-MFA), which is applicable to systems that are at metabolic steady ...
Lara J, Jazmin, Jamey D, Young
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2014
Metabolic Flux Analysis: Methods and Protocols opens up the field of metabolic flux analysis to those who want to start a new flux analysis project but are overwhelmed by the complexity of the approach. Metabolic flux analysis emerged from the current limitation for the prediction of metabolic fluxes from a measured inventory of the cell.
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Metabolic Flux Analysis: Methods and Protocols opens up the field of metabolic flux analysis to those who want to start a new flux analysis project but are overwhelmed by the complexity of the approach. Metabolic flux analysis emerged from the current limitation for the prediction of metabolic fluxes from a measured inventory of the cell.
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Application of Metabolic Flux Analysis in Metabolic Engineering
2011Metabolic flux analysis (MFA) is an important analytical technique to quantify intracellular metabolic fluxes as a consequence of all catalytic and transcriptional interactions. In systems metabolic engineering, MFA has played important role to understand cellular physiology under particular conditions and predict its metabolic capability after genetic
Lee, SY Lee, Sang-Yup +2 more
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Metabolic flux analysis in mammalian cell culture
Metabolic Engineering, 2010Mammalian cell culture metabolism is characterized by glucoglutaminolysis, that is, high glucose and glutamine uptake combined with a high rate of lactate and non-essential amino acid secretion. Stress associated with acid neutralization and ammonia accumulation necessitates complex feeding schemes and limits cell densities achieved in fed-batch ...
Quek, Lake-Ee +3 more
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Metabolic Flux Analysis of Postburn Hepatic Hypermetabolism
Metabolic Engineering, 2000The hepatic response to severe injury is characterized by a marked upregulation of glucose, fatty acid, and amino acid turnover, which, if persistent, predisposes the patient to progressive organ dysfunction. To study the effect of injury on liver intermediary metabolism, metabolic flux analysis was applied to isolated perfused livers of burned and ...
K, Lee +4 more
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