Results 61 to 70 of about 284,867 (287)
Peptide‐based ligand antagonists block a Vibrio cholerae adhesin
FEBS Letters, EarlyView.The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang +9 more
wiley +1 more source
Analysis of Metabolic Subnetworks by Flux Cone Projection
Algorithms for Molecular Biology, 2012 Background Analysis of elementary modes (EMs) is proven to be a powerful constraint-based method in the study of metabolic networks. However, enumeration of EMs is a hard computational task.
Marashi Sayed-Amir +2 more
doaj +1 more source
Reaction lumping in metabolic networks for application with thermodynamic metabolic flux analysis
Scientific Reports, 2021 Thermodynamic metabolic flux analysis (TMFA) can narrow down the space of steady-state flux distributions, but requires knowledge of the standard Gibbs free energy for the modelled reactions.
Lea Seep +2 more
doaj +1 more source
Metabolic signatures of regulation by phosphorylation and acetylation
iScience, 2022 Summary: Acetylation and phosphorylation are highly conserved posttranslational modifications (PTMs) that regulate cellular metabolism, yet how metabolic control is shared between these PTMs is unknown. Here we analyze transcriptome, proteome, acetylome,
Kirk Smith +3 more
doaj +1 more source
An improved multi-parametric programming algorithm for flux balance analysis of metabolic networks
, 2018 Flux balance analysis has proven an effective tool for analyzing metabolic networks. In flux balance analysis, reaction rates and optimal pathways are ascertained by solving a linear program, in which the growth rate is maximized subject to mass-balance ...
Akbari, Amir, Barton, Paul I.
core +1 more source
Quantification of folate metabolism using transient metabolic flux analysis [PDF]
Cancer & Metabolism, 2015 Systematic quantitative methodologies are needed to understand the heterogeneity of cell metabolism across cell types in normal physiology, disease, and treatment. Metabolic flux analysis (MFA) can be used to infer steady state fluxes, but it does not apply for transient dynamics.
Tedeschi, P.M. +8 more
openaire +4 more sources
Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer
FEBS Letters, EarlyView.Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani +4 more
wiley +1 more source
iScience, 2022 Summary: The study of cellular metabolism is limited by the amount of experimental data available. Formulations able to extract relevant predictions from accessible measurements are needed.
José Antonio Pereiro-Morejón +2 more
doaj +1 more source
Mechanisms of parasite‐mediated disruption of brain vessels
FEBS Letters, EarlyView.Parasites can affect the blood vessels of the brain, often causing serious neurological problems. This review explains how different parasites interact with and disrupt these vessels, what this means for brain health, and why these processes matter. Understanding these mechanisms may help us develop better ways to prevent or treat brain infections in ...
Leonor Loira +3 more
wiley +1 more source
iScienceSummary: Dietary restriction of the sulfur-containing amino acids methionine and cysteine (SAAR) has numerous metabolic benefits including enhanced body composition and insulin sensitivity.
Charlotte G. Mann +13 more
doaj +1 more source