Results 81 to 90 of about 1,002,608 (307)

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Air volume not spray concentration determines in vivo efficacy of volatile organic compounds against Plasmopara viticola

Scientific Reports
Volatile organic compounds (VOCs) are produced by plants in response to abiotic and biotic stress stimuli. Two possible modes of action against plant pathogens have been reported for VOCs, such as the induction of plant resistance and direct inhibition ...
Sabine Oberhofer, Sara Avesani, Michele Perazzolli, Peter Robatscher, Urban Spitaler   +4 more
doaj   +1 more source

Identification of the first plant caffeoyl-quinate esterases in Cichorium intybus

Frontiers in Plant Science
Chlorogenic acid (5-CQA) is a caffeic acid ester widely accumulated in higher plants. It plays roles in defense against biotic and abiotic stresses. As its biosynthetic pathway shares common enzymes and intermediates with that of lignin, 5-CQA has long ...
Antoine Mallavergne, David Mathiron, Roland Molinié, Jean-Louis Hilbert, David Gagneul   +4 more
doaj   +1 more source

Cloning and characterization of novel methylsalicylic acid synthase gene involved in the biosynthesis of isoasperlactone and asperlactone in Aspergillus westerdijkiae [PDF]

, 2009
Aspergillus westerdijkiae is the main producer of several biologically active polyketide metabolites including isoasperlactone and asperlactone. A 5298 bp polyketide synthase gene ‘‘aomsas” has been cloned in Aspergillus westerdijkiae by using gene ...
Atoui, Ali, Dobson, Alan D. W., Mathieu, Florence, Puel, Olivier, Dao, Huy Phong, Liboz, Thierry, O’Callaghan, John, O'Callaghan, John, Bacha, Nafees, Dobson, Alan D.W., Lebrihi, Ahmed   +10 more
core   +1 more source

Time‐restricted feeding prior to Mycobacterium tuberculosis infection reduces tissue CD4+ T cells with limited impact on bacterial clearance

FEBS Open Bio, EarlyView.
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta, Nidhi Yadav, Subhasmita Das, R. Rajendra Kumar Reddy, Nupur Sharma, Amol Ratnakar Suryawanshi, Jaswinder Singh Maras, Ranjan Kumar Nanda   +7 more
wiley   +1 more source

Upcycling of By-Products from Autochthonous Red Grapes and Commercial Apples as Ingredients in Baked Goods: A Comprehensive Study from Processing to Consumer Consumption

Antioxidants
Lagrein grape (Vitis vinifera L.) pomace and Scilate apple (Malus domestica Borkh.) skin are polyphenol- and antioxidant-rich by-products with promising applications in the food industry. This study investigated the impact of drying and grinding on their
Gaetano Cardone, Martina Magni, Veronica Marin, Andrea Pichler, Daniele Zatelli, Peter Robatscher, Ombretta Polenghi, Virna Lucia Cerne, Michael Oberhuber, Silvano Ciani   +9 more
doaj   +1 more source

The development of a solid-phase fermentation system for the production of pharmaceutically useful secondary metabolites in fungi [PDF]

, 2000
A Phoma species, producing a squalestatin (Si) was grown on agar media derived from wheat, oats, oil seed rape and malt extract over a range of water availability values corresponding to water activity (aa) levels of. 0.998,0.995,0.990,0.980 and 0.960.
Aldred, David
core  

Cyclic azapeptide CD36 ligand attenuates cardiac injury and reduces long‐chain fatty acid accumulation after myocardial ischemia–reperfusion in mice

FEBS Open Bio, EarlyView.
In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin, Naghme Radmannia, David N. Huynh, Liliane Ménard, Caroline Daneault, Maïté Veilleux, Ahsanullah Ahsanullah, André C. Carpentier, William D. Lubell, Matthieu Ruiz, Huy Ong, Sylvie Marleau, Simon‐Pierre Gravel   +12 more
wiley   +1 more source

HPLC assay of zearalenone and reduced metabolites in S9 fractions of duck liver [PDF]

, 2007
HPLC analysis of zearalenone (ZEA), zearalenols (-ZOL and ß-ZOL) and zearalanols (-ZAL and ß-ZAL) was developed, in order to obtain a sensitive and reproducible method to quantify ZEA and its reduced metabolites in subcellular fractions of animal livers (
Guerre, Philippe, Kolf-Clauw, Martine, Tardieu, Didier, Ayouni, Fatma   +3 more
core