Results 41 to 50 of about 12,952 (205)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Microwave and Ultrasound Augmented Leaching of Complicated Zinc Oxide Ores in Ammonia and Ammonium Citrate Solutions

Metals, 2017
Recovery of zinc from low grade zinc oxide ore is attempted with ammonia and ammonium citrate solutions augmented by microwave roasting and ultrasound radiation.
Libo Zhang, Haoyu Li, Jinhui Peng, Chandrasekar Srinivasakannan, Shiwei Li, Shaohua Yin   +5 more
doaj   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Removal of Antimony from Industrial Crude Arsenic by Vacuum Sublimation: Combination of Thermodynamics and Ab Initio Molecular Dynamics

Metals
Thermodynamic theory was employed in this study to investigate the feasibility of separating antimony (Sb) from crude arsenic (As) using vacuum sublimation.
Zibin Zuo, Mengping Duan, Xinyang Liu, Xiumin Chen, Huan Luo, Tengteng Shi, Xianjun Lei, Yang Tian, Bin Yang, Baoqiang Xu   +9 more
doaj   +1 more source

The crystal structure of the Borrelia burgdorferi nicotinamidase BBE22 resolves a long‐standing annotation error

FEBS Open Bio, EarlyView.
The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Microwave-assisted and regular leaching of germanium from the germanium-rich lignite ash

Green Processing and Synthesis, 2018
Demand for germanium (Ge) is rapidly growing in recent years in various fields including semiconductors, aerospace, and solar cells. However, the Ge resources are very limited in the world.
Chen Yuqian, Zhou Junwen, Zhang Libo, Peng Jinhui, Li Shiwei, Yin Shaohua, Yang Kun, Lin Yaping   +7 more
doaj   +1 more source

Magnesium production by carbothermic reduction in vacuum

Journal of Magnesium and Alloys, 2015
In present work, we investigate production of magnesium by carbothermic reduction under vacuum conditions. The process was divided into two parts, one is reduction process, and the other one is condensation process. The experimental results revealed that
Yang Tian, Bao-qiang Xu, Bin Yang, Cheng-b Yang, Tao Qu, Da-chun Liu, Yong-nian Dai   +6 more
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Preparation of Small-Sized and Uniformly Distributed SnO by Ultrasound at Room Temperature

Metals
A novel ultrasound-assisted method for synthesizing small, uniform stannous oxide (SnO) at room temperature was proposed in this work. The experimental results showed that the median particle size D50 of SnO prepared by ultrasound was 5.2 μm, with a ...
Mingge Fu, Liuxin Xiang, Qian Zhang, Tao Xv, Thiquynhxuan Le, Libo Zhang   +5 more
doaj   +1 more source