Results 51 to 60 of about 7,983,382 (305)

The crystal structure of the Borrelia burgdorferi nicotinamidase BBE22 resolves a long‐standing annotation error

FEBS Open Bio, EarlyView.
The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley   +1 more source

Catalytic Methane Decomposition for the Simultaneous Production of Hydrogen and Low-Reactivity Biocarbon for the Metallurgic Industry

Energies
To reach agreed-on climate goals, it is necessary to develop new energy carriers and industrial materials that are carbon-neutral. To combat global warming and keep Earth’s temperature from increasing by 1.5 °C, some of these solutions need to be carbon ...
Roger A. Khalil, Sethulakshmy Jayakumari, Halvor Dalaker, Liang Wang, Pål Tetlie, Øyvind Skreiberg   +5 more
doaj   +1 more source

Investigations of Plasticity of Hard-Deformed Aluminium Alloys of 5xxx Series Using Torsion Plastometer

Archives of Metallurgy and Materials, 2016
This paper presents the results of the study aimed at determination of plasticity of selected grades of hard-deformed aluminium alloys of 5xxx series in terms of parameters characteristic for the process of extrusion.
Laber K., Kawałek A., Sawicki S., Dyja H., Borowski J., Leśniak D., Jurczak H.   +6 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Seramik- Metal Soğuk Birleştirmeler Ve Performanslarının İncelenmesi [PDF]

, 2007
06.03.2018 tarihli ve 30352 sayılı Resmi Gazetede yayımlanan “Yükseköğretim Kanunu İle Bazı Kanun Ve Kanun Hükmünde Kararnamelerde Değişiklik Yapılması Hakkında Kanun” ile 18.06.2018 tarihli “Lisansüstü Tezlerin Elektronik Ortamda Toplanması ...
Yardımcı Doçent Doktor Ramazan Kayıkçı ; Yrd. Doç. Dr. Ali Osman Kurt, Sakarya Üniversitesi, Fen Bilimleri Enstitüsü, Metal Eğitimi Anabilim Dalı, Metal Eğitimi, Ünal, İbrahim   +2 more
core  

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

The Role of Carbon in the Mechanism of Ferritic-Austenitic Cast Steel Solidification

Archives of Foundry Engineering, 2014
The paper presents the results of research on the microstructure of GX2CrNiMoCuN25-6-3-3 and GX2CrNiMoCuN25-6-3 cast steels with a varying carbon content.
Stradomski G.
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source