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Metagenomic Applications to Herbivore Gut Microbiomes: A Comprehensive Review of Microbial Diversity and Host Interactions. [PDF]
Wei J +8 more
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Ammonium-Generating Microbial Consortia in Paddy Soil Revealed by DNA-Stable Isotope Probing and Metatranscriptomics. [PDF]
Wang CN, Masuda Y, Senoo K.
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Deciphering Metatranscriptomic Data
2014Metatranscriptomic data contributes another piece of the puzzle to understanding the phylogenetic structure and function of a community of organisms. High-quality total RNA is a bountiful mixture of ribosomal, transfer, messenger and other noncoding RNAs, where each family of RNA is vital to answering questions concerning the hidden microbial world ...
Kopylova, Evguenia +6 more
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Quantitative Microbial Metatranscriptomics
2014The direct retrieval and sequencing of environmental RNA is emerging as a powerful technique to elucidate the in situ activities of microbial communities. Here we provide a metatranscriptomic protocol describing environmental sample collection, rRNA depletion, mRNA amplification, cDNA synthesis, and bioinformatic analysis.
Scott, Gifford +2 more
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Gene Expression Profiling: Metatranscriptomics
2011Metatranscriptomics has been developed to help understand how communities respond to changes in their environment. Metagenomic studies provided a snapshot of the genetic composition of the community at any given time. However, short-timescale studies investigating the response of communities to rapid environmental changes (e.g.
Jack A, Gilbert, Margaret, Hughes
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Metatranscriptomics of Soil Eukaryotic Communities
2016Functions expressed by eukaryotic organisms in soil can be specifically studied by analyzing the pool of eukaryotic-specific polyadenylated mRNA directly extracted from environmental samples. In this chapter, we describe two alternative protocols for the extraction of high-quality RNA from soil samples.
Yadav, Rajiv +4 more
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Application of metatranscriptomics to soil environments
Journal of Microbiological Methods, 2012The activities of soil microbial communities are of critical importance to terrestrial ecosystem functioning. The mechanisms that determine the interactions between soil microorganisms, their environment and neighbouring organisms, however, are poorly understood.
Carvalhais, Lilia C. +3 more
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