Results 21 to 30 of about 288 (110)

High complexity of Glutamine synthetase regulation in Methanosarcina mazei: Small protein 26 interacts and enhances glutamine synthetase activity

open access: yesThe FEBS Journal, Volume 288, Issue 18, Page 5350-5373, September 2021., 2021
Several small proteins have been recently identified in Methanosarcina mazei. By functional and biochemical characterizations, we demonstrated that the small protein sP26 interacts with the glutamine synthetase under nitrogen starvation and enhances its activity by stabilizing the dodecameric structure independently and in addition to the known ...
Miriam Gutt   +11 more
wiley   +1 more source

Thermal adaptation of the archaeal and bacterial lipid membranes. [PDF]

open access: yesArchaea, 2012
The physiological characteristics that distinguish archaeal and bacterial lipids, as well as those that define thermophilic lipids, are discussed from three points of view that (1) the role of the chemical stability of lipids in the heat tolerance of thermophilic organisms: (2) the relevance of the increase in the proportion of certain lipids as the ...
Koga Y.
europepmc   +2 more sources

The CARF Protein MM_0565 Affects Transcription of the Casposon-Encoded cas1-solo Gene in Methanosarcina mazei Gö1

open access: yesBiomolecules, 2020
Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) loci are found in bacterial and archaeal genomes where they provide the molecular machinery for acquisition of immunity against foreign DNA.
Andrea Ulbricht   +6 more
doaj   +1 more source

Diversity of the DNA Replication System in the Archaea Domain

open access: yesArchaea, Volume 2014, Issue 1, 2014., 2014
The precise and timely duplication of the genome is essential for cellular life. It is achieved by DNA replication, a complex process that is conserved among the three domains of life. Even though the cellular structure of archaea closely resembles that of bacteria, the information processing machinery of archaea is evolutionarily more closely related ...
Felipe Sarmiento   +4 more
wiley   +1 more source

Development of β‐Lactamase as a Tool for Monitoring Conditional Gene Expression by a Tetracycline‐Riboswitch in Methanosarcina acetivorans

open access: yesArchaea, Volume 2014, Issue 1, 2014., 2014
The use of reporter gene fusions to assess cellular processes such as protein targeting and regulation of transcription or translation is established technology in archaeal, bacterial, and eukaryal genetics. Fluorescent proteins or enzymes resulting in chromogenic substrate turnover, like β‐galactosidase, have been particularly useful for microscopic ...
Shemsi Demolli   +6 more
wiley   +1 more source

Genetic Confirmation of the Role of Sulfopyruvate Decarboxylase in Coenzyme M Biosynthesis in Methanococcus maripaludis

open access: yesArchaea, Volume 2013, Issue 1, 2013., 2013
Coenzyme M is an essential coenzyme for methanogenesis. The proposed biosynthetic pathway consists of five steps, of which the fourth step is catalyzed by sulfopyruvate decarboxylase (ComDE). Disruption of the gene comE by transposon mutagenesis resulted in a partial coenzyme M auxotroph, which grew poorly in the absence of coenzyme M and retained less
Felipe Sarmiento   +3 more
wiley   +1 more source

Compiling a versatile toolbox for inducible gene expression in Methanosarcina mazei. [PDF]

open access: yesMicrolife
Methanosarcina mazei is a model organism, providing a platform to explore methanoarchaeal regulation mechanisms on the transcriptional and translational level.
Hüttermann J, Schmitz R.
europepmc   +2 more sources

Laboratory tools for detection of archaea in humans [PDF]

open access: yes, 2012
This work represents an update of knowledge regarding the detection methods for human microbiome-associated archaea. Despite the fact that, during the last three decades, only four methanoarchaeal species have been isolated from the human mucosa ...
Dridi, B.
core   +1 more source

Establishing a Markerless Genetic Exchange System for Methanosarcina mazei Strain Gö1 for Constructing Chromosomal Mutants of Small RNA Genes

open access: yesArchaea, Volume 2011, Issue 1, 2011., 2011
A markerless genetic exchange system was successfully established in Methanosarcina mazei strain Gö1 using the hpt gene coding for hypoxanthine phosphoribosyltransferase. First, a chromosomal deletion mutant of the hpt gene was generated conferring resistance to the purine analog 8‐aza‐2,6‐diaminopurine (8‐ADP).
Claudia Ehlers   +3 more
wiley   +1 more source

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