Results 11 to 20 of about 10,534 (145)

Cold Orthogonal Translation: A Psychrophilic Pyrrolysyl-tRNA Synthetase Boosts Genetic Code Expansion in E. coli. [PDF]

open access: yesAdv Sci (Weinh)
ABSTRACT Orthogonal translation systems (OTSs) enable site‐specific incorporation of non‐canonical amino acids (ncAAs) and are central to genetic code expansion. Current engineering strategies typically rely on hyperstable aminoacyl tRNA synthetase (aaRS) scaffolds to tolerate destabilizing mutations required for substrate diversification.
Koch NG   +4 more
europepmc   +2 more sources

Differential regulation of Ota and Otb, two primary glycine betaine transporters in the methanogenic archaeon Methanosarcina mazei go1 [PDF]

open access: yes, 2008
Methanogenic archaea accumulate glycine betaine in response to hypersalinity, but the regulation of proteins involved, their mechanism of activation and regulation of the corresponding genes are largely unknown.
Schmidt, S.   +5 more
core   +1 more source

Conductive Particles Enable Syntrophic Acetate Oxidation between Geobacter and Methanosarcina from Coastal Sediments

open access: yesmBio, 2018
Coastal sediments are rich in conductive particles, possibly affecting microbial processes for which acetate is a central intermediate. In the methanogenic zone, acetate is consumed by methanogens and/or syntrophic acetate-oxidizing (SAO) consortia.
Amelia-Elena Rotaru   +10 more
doaj   +1 more source

Insights into pyrrolysine function from structures of a trimethylamine methyltransferase and its corrinoid protein complex

open access: yesCommunications Biology, 2023
Structures of Methanosarcina barkeri trimethylamine methyltransferase (MttB) with its substrates reveal the role of pyrrolysine in methyl group transfer from trimethylamine to the corrinoid cofactor in MttC.
Jiaxin Li   +6 more
doaj   +1 more source

Insights into the biotechnology potential of Methanosarcina

open access: yesFrontiers in Microbiology, 2022
Methanogens are anaerobic archaea which conserve energy by producing methane. Found in nearly every anaerobic environment on earth, methanogens serve important roles in ecology as key organisms of the global carbon cycle, and in industry as a source of ...
Sean Carr, Nicole R. Buan
doaj   +1 more source

Nonnative Thiol and Aniline Methylation, Including Enantioselective Dithiol Desymmetrization, by Multicomponent Cobamide‐Dependent Methyltransferases Using Unactivated Methyl Donors

open access: yesAngewandte Chemie, EarlyView.
Mixed MtaABC/MtgABC methyltransferase systems were developed for non‐native thiol and aniline methylation using the unactivated methyl donors methanol and glycine betaine. The platform enabled site‐selective and enantioselective methylation with turnover number up to 6000, representing the first enantioselective non‐native catalysis by cobamide ...
Amardeep Kumar   +3 more
wiley   +2 more sources

Metabolic Adaptation of Methanogens in Anaerobic Digesters Upon Trace Element Limitation

open access: yesFrontiers in Microbiology, 2018
Anaerobic digestion (AD) is a complex multi-stage process relying on the activity of highly diverse microbial communities including hydrolytic, acidogenic and syntrophic acetogenic bacteria as well as methanogenic archaea.
Babett Wintsche   +4 more
doaj   +1 more source

Necessity of electrically conductive pili for methanogenesis with magnetite stimulation [PDF]

open access: yesPeerJ, 2018
Background Magnetite-mediated direct interspecies electron transfer (DIET) between Geobacter and Methanosarcina species is increasingly being invoked to explain magnetite stimulation of methane production in anaerobic soils and sediments.
Oumei Wang   +4 more
doaj   +2 more sources

Intermittent fasting for microbes: how discontinuous feeding increases functional stability in anaerobic digestion

open access: yesBiotechnology for Biofuels, 2018
Background Demand-driven biogas production could play an important role for future sustainable energy supply. However, feeding a biogas reactor according to energy demand may lead to organic overloading and, thus, to process failures.
Fabian Bonk   +6 more
doaj   +1 more source

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