Results 101 to 110 of about 4,218 (198)

Nature AND nurture: enabling formate‐dependent growth in Methanosarcina acetivorans

The FEBS Journal, Volume 292, Issue 9, Page 2251-2271, May 2025.
We identified a cytosolic formate dehydrogenase (FdhAB) present in several Methanosarcinales, likely acquired by independent horizontal gene transfers. We engineered two strains of Methanosarcina acetivorans: (a) JB‐MF and (b) JB‐F2 by functionally expressing FdhAB.
Jichen Bao, Tejas Somvanshi, Yufang Tian, Maxime G. Laird, Pierre Simon Garcia, Christian Schöne, Michael Rother, Guillaume Borrel, Silvan Scheller   +8 more
wiley   +1 more source

Survival of microorganisms during two-year exposure in outer space near the ISS

Scientific Reports
Results of an experiment named "Test" on survival and variability of microorganisms in open space near the International Space Station are presented. It was found after two-years exposure, spore-forming bacteria of the species Bacillus subtilis, fungi of
Elena A. Deshevaya, Svetlana V. Fialkina, Elena V. Shubralova, Oleg S. Tsygankov, Natalia M. Khamidullina, Leonid M. Vasilyak, Vladimir Ya. Pecherkin, Viktoria A. Shcherbakova, Andrey M. Nosovsky, Оleg I. Orlov   +9 more
doaj   +1 more source

How Small Proteins Adjust the Metabolism of Cyanobacteria Under Stress

BioEssays, Volume 47, Issue 3, March 2025.
Cyanobacteria are powered by oxygenic photosynthesis located in extensive internal membrane systems, the thylakoids. Using carboxysomes, they assimilate organic from inorganic carbon. The integration of photosynthesis, carbon fixation, assimilation of nitrogen, and other nutrients involves several small proteins.
Alexander Kraus, Wolfgang R. Hess
wiley   +1 more source

Simplified methodology for a modular and genetically expanded protein synthesis in cell-free systems

Synthetic and Systems Biotechnology, 2019
Genetic code expansion, which enables the site-specific incorporation of unnatural amino acids into proteins, has emerged as a new and powerful tool for protein engineering.
Yonatan Chemla, Eden Ozer, Michael Shaferman, Ben Zaad, Rambabu Dandela, Lital Alfonta   +5 more
doaj   +1 more source

Regulation of DNA Topology in Archaea: State of the Art and Perspectives

Molecular Microbiology, Volume 123, Issue 3, Page 245-264, March 2025.
DNA topoisomerases are ubiquitous enzymes that play a crucial role in regulating DNA supercoiling which affects fundamental biological processes involving DNA. In this review, we summarize the current understanding of regulation of supercoiling by DNA topoisomerases in the third domain of life, the Archaea, with a particular focus on three key model ...
Paul Villain, Tamara Basta
wiley   +1 more source

sRNA₄₁ affects ribosome binding sites within polycistronic mRNAs in Methanosarcina mazei Gö1 [PDF]

Molecular Microbiology, 2018
SummarySeveral noncoding RNAs potentially involved in nitrogen (N)‐regulation have been detected in Methanosarcina mazei, however, targets have been identified only for one of them. Here, we report on the function of sRNA41, highly expressed under N‐sufficiency.
Anne Buddeweg, Kundan Sharma, Henning Urlaub, Ruth A. Schmitz   +3 more
openaire   +4 more sources

Tetrahydrofolat-spezifische Enzyme im Baustoffwechsel von Methanosarcina barkeri sowie die Rolle von Folsäure und p-Aminobenzoesäure als Wachstumsfaktoren in diesem Archaeon [PDF]

, 2004
Tetrahydrofolat (H4F) spielt als Überträger von C1-Einheiten im Baustoffwechsel von allen Bacteria und Eucarya und von einigen Archaea eine wichtige Rolle in der Synthese von C1-Einheiten enthaltenden Verbindungen wie Purine, Thymidin und Methionin.
Buchenau, Bärbel, Thauer, Rudolf K. (Prof. Dr.)   +1 more
core   +1 more source

Light‐Activatable Ubiquitin for Studying Linkage‐Specific Ubiquitin Chain Formation Kinetics

Advanced Science, Volume 12, Issue 6, February 10, 2025.
Ubiquitin dynamically forms differently linked polyubiquitin chains. To study rapid cellular kinetics of global linkage‐specific ubiquitination, light‐activatable polyubiquitin chain formation is reported via incorporation of a photocaged lysine. Minute‐scale ubiquitination kinetics for different linkages are observed, and the impact of ubiquitination ...
Sudakshina Banerjee, Zeyneb Vildan Cakil, Kai Gallant, Johannes van den Boom, Shubhendu Palei, Hemmo Meyer, Malte Gersch, Daniel Summerer   +7 more
wiley   +1 more source

Microbial populations associated with fixed- and floating-bed reactors during a two-stage anaerobic process [PDF]

, 2008
Microbial populations associated with methanogenic fixed- or floating-bed bioreactors used for anaerobic digestion of lignocellulosic waste were investigated.
Martina Hausner, Neena Raizada, Peter A. Wilderer, Vikas Sonakya   +3 more
core   +2 more sources

Selection and assembly of indigenous bacteria and methanogens from spent metalworking fluids and their potential as a starting culture in a fluidized bed reactor

Microbial Biotechnology, 2019
Summary Waste metalworking fluids (MWFs) are highly biocidal resulting in real difficulties in the, otherwise favoured, bioremediation of these high chemical oxygen deman (COD) wastes anaerobically in bioreactors.
Ioannis Vyrides, Damian W. Rivett, Kenneth D. Bruce, Andrew K. Lilley   +3 more
doaj   +1 more source