Therapeutic applications of genetic code expansion [PDF]
Synthetic and Systems Biotechnology, 2018 In nature, a limited, conservative set of amino acids are utilized to synthesize proteins. Genetic code expansion technique reassigns codons and incorporates noncanonical amino acids (ncAAs) through orthogonal aminoacyl-tRNA synthetase (aaRS)/tRNA pairs.
Yujia Huang, Tao Liu
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tRNA engineering strategies for genetic code expansion [PDF]
Frontiers in GeneticsThe advancement of genetic code expansion (GCE) technology is attributed to the establishment of specific aminoacyl-tRNA synthetase/tRNA pairs. While earlier improvements mainly focused on aminoacyl-tRNA synthetases, recent studies have highlighted the ...
YouJin Kim +3 more
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Recent Development of Genetic Code Expansion for Posttranslational Modification Studies
Molecules, 2018 Nowadays advanced mass spectrometry techniques make the identification of protein posttranslational modifications (PTMs) much easier than ever before. A series of proteomic studies have demonstrated that large numbers of proteins in cells are modified by
Hao Chen +4 more
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Improving the Efficiency and Orthogonality of Genetic Code Expansion [PDF]
BioDesign Research, 2022 The site-specific incorporation of the noncanonical amino acid (ncAA) into proteins via genetic code expansion (GCE) has enabled the development of new and powerful ways to learn, regulate, and evolve biological functions in vivo.
Xian Fu, Yijian Huang, Yue Shen
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Ribosome engineering enhances genetic code expansion in Saccharomyces cerevisiae [PDF]
Synthetic and Systems BiotechnologyGenetic code expansion enables the site-specific installation of noncanonical amino acids (ncAAs) into proteins, but its limited efficiency in eukaryotes remains a major barrier to broader application.
Xiaoxu Chen +6 more
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Use of non‐canonical amino acids in genetic code expansion‐based therapeutics: Effects on mouse gut microbiota [PDF]
Microbial Biotechnology, 2023 Vaccines and cell therapeutics based on genetic code expansion are emerging. A crucial step in these therapeutic technologies is the oral administration of non‐canonical amino acids (ncAAs) to control pathogen growth and therapeutic protein levels in ...
Manyu Liang +7 more
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Applications of genetic code expansion and photosensitive UAAs in studying membrane proteins [PDF]
Open Life Sciences, 2023 Membrane proteins are the targets for most drugs and play essential roles in many life activities in organisms. In recent years, unnatural amino acids (UAAs) encoded by genetic code expansion (GCE) technology have been widely used, which endow proteins ...
Zhao Shu, Liu Dong
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Engineering a light-controllable fluorescent protein for peroxynitrite detection via genetic code expansion [PDF]
RSC Chemical BiologyWe report a noncanonical amino acid-containing photoconvertible fluorescent protein for selective peroxynitrite detection. Incorporation of p-borono-l-phenylalanine into circularly permuted mEos2 affords a peroxynitrite-responsive sensor that retains ...
Gloricelly M. Roman Arocho +5 more
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Suppressor tRNAs at the interface of genetic code expansion and medicine [PDF]
Frontiers in GeneticsSuppressor transfer RNAs (sup-tRNAs) are receiving renewed attention for their promising therapeutic properties in treating genetic diseases caused by nonsense mutations.
Aya Awawdeh +2 more
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Remodeling the cellular stress response for enhanced genetic code expansion in mammalian cells [PDF]
Nature Communications, 2023 Genetic code expansion (GCE) reprograms the translational machinery to site-specifically incorporate noncanonical amino acids (ncAAs) into a selected protein.
Mikhail E. Sushkin +2 more
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