Results 21 to 30 of about 4,608,949 (263)
A robust platform streamlining aromatic noncanonical amino acid biosynthesis and genetic code expansion in Escherichia coli [PDF]
Nature CommunicationsGenetic code expansion (GCE) has significantly enhanced the diversity of proteins in the biological world, leading to a wide range of applications.
Jingxuan Zhang +8 more
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Reversing the charge of lysine by genetic code expansion
Nature ChemistryPosttranslational modifications alter the structure and function of proteins. Now, genetic code expansion enables encoding of ε-N-succinyllysine and ε-N-glutaryllysine residues to decipher the effects of these modifications on enzymatic activity, protein–
Olsen, Christian A., Danková, Daniela
core +4 more sources
Genetic Code Expansion: Another Solution to Codon Assignments. [PDF]
Int J Mol Sci, 2022 This Special Issue is intended to highlight recent advances in genetic code expansion, particularly the site-specific incorporation of noncanonical amino acids (ncAAs) into proteins [...
Sakamoto K.
europepmc +2 more sources
Site-specific dual encoding and labeling of proteins via genetic code expansion. [PDF]
Cell Chem Biol, 2023 Bednar RM, Karplus PA, Mehl RA.
europepmc +2 more sources
Genetic Code Expansion in Pseudomonas putida KT2440. [PDF]
ACS Synth Biol, 2022 Pseudomonas putida KT2440 is an emerging microbial chassis for biobased chemical production from renewable feedstocks and environmental bioremediation. However, tools for studying, engineering, and modulating protein complexes and biosynthetic enzymes in
He X +5 more
europepmc +2 more sources
Expansion of the genetic code via expansion of the genetic alphabet [PDF]
Current Opinion in Chemical Biology, 2018 Current methods to expand the genetic code enable site-specific incorporation of non-canonical amino acids (ncAAs) into proteins in eukaryotic and prokaryotic cells. However, current methods are limited by the number of codons possible, their orthogonality, and possibly their effects on protein synthesis and folding.
Vivian T Dien +3 more
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Genetic Code Expansion in Animals [PDF]
ACS Chemical Biology, 2018 Expanding the genetic code to enable the incorporation of unnatural amino acids into proteins in biological systems provides a powerful tool for studying protein structure and function. While this technology has been mostly developed and applied in bacterial and mammalian cells, it recently expanded into animals, including worms, fruit flies, zebrafish,
Wes Brown, Jihe Liu, Alexander Deiters
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Genetic Code Expansion: A Brief History and Perspective [PDF]
Biochemistry, 2021 Since the establishment of site-specific mutagenesis of single amino acids to interrogate protein function in the 1970s, biochemists have sought to tailor protein structure in the native cell environment. Fine-tuning the chemical properties of proteins is an indispensable way to address fundamental mechanistic questions.
Mia A. Shandell +2 more
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Genetic Code Expansion Approaches to Decipher the Ubiquitin Code. [PDF]
Chem RevISSN:0009 ...
Wanka V, Fottner M, Cigler M, Lang K.
europepmc +4 more sources
Genetic code expansion in the mouse brain [PDF]
Nature Chemical Biology, 2016 Site-specific incorporation of non-natural amino acids into proteins, via genetic code expansion with pyrrolysyl tRNA synthetase (PylRS) and tRNA(Pyl)CUA pairs (and their evolved derivatives) from Methanosarcina sp., forms the basis of powerful approaches to probe and control protein function in cells and invertebrate organisms.
Russell J Ernst +8 more
openaire +2 more sources