Results 11 to 20 of about 4,608,949 (263)
The Role of Orthogonality in Genetic Code Expansion [PDF]
Life, 2019 The genetic code defines how information in the genome is translated into protein. Aside from a handful of isolated exceptions, this code is universal. Researchers have developed techniques to artificially expand the genetic code, repurposing codons and ...
Pol Arranz-Gibert +2 more
doaj +4 more sources
Engineering Pyrrolysine Systems for Genetic Code Expansion and Reprogramming. [PDF]
Chem RevOver the past 16 years, genetic code expansion and reprogramming in living organisms has been transformed by advances that leverage the unique properties of pyrrolysyl-tRNA synthetase (PylRS)/tRNAPyl pairs.
Dunkelmann DL, Chin JW.
europepmc +9 more sources
Genetic Code Expansion of Vibrio natriegens [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 Escherichia coli has been considered as the most used model bacteria in the majority of studies for several decades. However, a new, faster chassis for synthetic biology is emerging in the form of the fast-growing gram-negative bacterium Vibrio ...
Eden Ozer, Lital Alfonta
doaj +3 more sources
Generalization of Genetic Code Expansion
, 2021 The standard genetic code directs the assembly of the 20 standard amino acids into proteins and defines function in biology. Through the central dogma, DNA is transcribed into RNA which is translated by the well-understood machinery of the ribosome and ...
Stork, Devon
core +6 more sources
Establishing the fundamental rules for genetic code expansion. [PDF]
Nat Chem, 2023 Genetic code expansion beyond α-amino acids is a major challenge, in which stitching non-natural building blocks within the ribosome is a critical barrier.
Sinha S, Ahsan M, Palermo G.
europepmc +6 more sources
Studying Acetylation of Aconitase Isozymes by Genetic Code Expansion [PDF]
Frontiers in Chemistry, 2022 Aconitase catalyzes the second reaction of the tricarboxylic acid cycle, the reversible conversion of citrate and isocitrate. Escherichia coli has two isoforms of aconitase, AcnA and AcnB. Acetylomic studies have identified acetylation at multiple lysine
Jessica Araujo +5 more
doaj +2 more sources
Metalloprotein design using genetic code expansion
Chem. Soc. Rev., 2014 More than one third of all proteins are metalloproteins. They catalyze important reactions such as photosynthesis, nitrogen fixation and CO2 reduction. Metalloproteins such as the olfactory receptors also serve as highly elaborate sensors. Here we review
Yu, Y. +4 more
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Reading and Writing the Ubiquitin Code Using Genetic Code Expansion. [PDF]
ChembiochemAbstractDeciphering ubiquitin proteoform signaling and its role in disease has been a long‐standing challenge in the field. The effects of ubiquitin modifications, its relation to ubiquitin‐related machineries, and its signaling output has been particularly limited by its reconstitution and means of characterization.
Patel RS, Pannala NM, Das C.
europepmc +4 more sources
Genetic Code Expansion in Mammalian Cells [PDF]
, 2019 Proteins in nature are synthesized from a conservative set of 20 canonical amino acids, limiting the chemical space of biological systems. Over the last few decades, scientists have developed methods to expand the genetic code of living organisms ...
core +4 more sources
Genetic code expansion reveals site-specific lactylation in living cells reshapes protein functions [PDF]
Nature CommunicationsProtein lactylation is an emerging field. To advance the exploration of its biological functions, here we develop a comprehensive workflow that integrates proteomics to identify lactylated sites, genetic code expansion (GCE) for the expression of site ...
Chang Shao +15 more
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