Results 31 to 40 of about 4,608,949 (263)
An Amino Acid-Swapped Genetic Code [PDF]
, 2020 Preventing the escape of hazardous genes from genetically modified organisms (GMOs) into the environment is one of the most important issues in biotechnology research.
Tozaki, Masahiro +2 more
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Using Genetic Code Expansion for Protein Biochemical Studies
Frontiers in Bioengineering and Biotechnology, 2020 Protein identification has gone beyond simply using protein/peptide tags and labeling canonical amino acids. Genetic code expansion has allowed residue- or site-specific incorporation of non-canonical amino acids into proteins. By taking advantage of the
Christina Z. Chung +3 more
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Frontiers in Molecular Biosciences, 2023 Post-translational modifications (PTMs) can occur on almost all amino acids in eukaryotes as a key mechanism for regulating protein function. The ability to study the role of these modifications in various biological processes requires techniques to ...
Pearl Morosky +8 more
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Extensive breaking of genetic code degeneracy with non-canonical amino acids
Nature Communications, 2023 Genetic code expansion (GCE) offers many exciting opportunities for the creation of synthetic organisms and for drug discovery methods that utilize in vitro translation.
Clinton A. L. McFeely +5 more
doaj +1 more source
An Ecological Basis for Dual Genetic Code Expansion in Marine Deltaproteobacteria
Frontiers in Microbiology, 2021 Marine benthic environments may be shaped by anthropogenic and other localized events, leading to changes in microbial community composition evident decades after a disturbance.
Veronika Kivenson +2 more
doaj +1 more source
Unconventional genetic code systems in archaea
Frontiers in Microbiology, 2022 Archaea constitute the third domain of life, distinct from bacteria and eukaryotes given their ability to tolerate extreme environments. To survive these harsh conditions, certain archaeal lineages possess unique genetic code systems to encode either ...
Kexin Meng +4 more
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Inducible Genetic Code Expansion in Eukaryotes
ChemBioChem, 2020 AbstractGenetic code expansion (GCE) is a versatile tool to site‐specifically incorporate a noncanonical amino acid (ncAA) into a protein, for example, to perform fluorescent labeling inside living cells. To this end, an orthogonal aminoacyl‐tRNA‐synthetase/tRNA (RS/tRNA) pair is used to insert the ncAA in response to an amber stop codon in the protein
Christine Koehler +3 more
openaire +2 more sources
Next-generation genetic code expansion [PDF]
Current Opinion in Chemical Biology, 2018 Engineering of the translation apparatus has permitted the site-specific incorporation of nonstandard amino acids (nsAAs) into proteins, thereby expanding the genetic code of organisms. Conventional approaches have focused on porting tRNAs and aminoacyl-tRNA synthetases (aaRS) from archaea into bacterial and eukaryotic systems where they have been ...
Pol, Arranz-Gibert +2 more
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Frontiers in Molecular Biosciences, 2022 Amino acid sequences of proteins are encoded in nucleic acids composed of four letters, A, G, C, and T(U). However, this four-letter alphabet coding system limits further functionalities of proteins by the twenty letters of amino acids.
Michiko Kimoto, Ichiro Hirao
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On the origin of the mitochondrial genetic code: Towards a unified mathematical framework for the management of genetic information [PDF]
, 2012 The origin of the genetic code represents one of the most challenging problems in molecular evolution. The genetic code is an important universal feature of extant organisms and indicates a common ancestry of different forms of life on earth.
Diego Luis Gonzalez +5 more
core +1 more source