Molecular assessment of complex microbial communities degrading long chain fatty acids in methanogenic bioreactors [PDF]
Microbial diversity of anaerobic sludge after extended contact with long chain fatty acids (LCFA) was studied using molecular approaches. Samples containing high amounts of accumulated LCFA were obtained after continuous loading of two bioreactors ...
Alfons J.M. Stams +58 more
core +2 more sources
A snapshot love story: what serial crystallography has done and will do for us
This article gives an overview of current trends and challenges in serial crystallography, with a focus on results from time‐resolved experiments.Serial crystallography, born from groundbreaking experiments at the Linac Coherent Light Source in 2009, has evolved into a pivotal technique in structural biology.
Alessandra Henkel, Dominik Oberthür
wiley +1 more source
Genetic, Biochemical, and Molecular Characterization of Methanosarcina barkeri Mutants Lacking Three Distinct Classes of Hydrogenase [PDF]
The methanogenic archaeon Methanosarcina barkeri encodes three distinct types of hydrogenase, whose functions vary depending on the growth substrate. These include the F_(420)-dependent (Frh), methanophenazine-dependent (Vht), and ferredoxin-dependent ...
Kulkarni, Gargi +2 more
core +1 more source
Functional and structural characterization of the Methanosarcina mazei proteasome and PAN complexes
We have cloned the proteasome and the proteasome activating nucleotidase (PAN) genes from the mesophilic archaeon Methanosarcina mazei and produced the respective proteins in Escherichia coli cultures. The recombinant complexes were purified to homogeneity and characterized biochemically, structurally, and by mass spectrometry.
Medalia, N. +6 more
openaire +4 more sources
Genome sequence of Acetomicrobium hydrogeniformans OS1 [PDF]
Acetomicrobium hydrogeniformans, an obligate anaerobe of the phylum Synergistetes, was isolated from oil production water. It has the unusual ability to produce almost 4 molecules H2/molecule glucose. The draft genome of A.
Cook, Lauren E +8 more
core +2 more sources
Evaluation and optimization of PCR primers for selective and quantitative detection of marine ANME subclusters involved in sulfate-dependent anaerobic methane oxidation [PDF]
Since the discovery that anaerobic methanotrophic archaea (ANME) are involved in the anaerobic oxidation of methane coupled to sulfate reduction in marine sediments, different primers and probes specifically targeting the 16S rRNA gene of these archaea ...
A Boetius +38 more
core +1 more source
We report here a comparative analysis of the genome sequence of Methanosarcina barkeri with those of Methanosarcina acetivorans and Methanosarcina mazei. The genome of M. barkeri is distinguished by having an organization that is well conserved with respect to the other Methanosarcina spp.
Maeder, Dennis L. +10 more
openaire +3 more sources
Genome-wide gene expression and RNA half-life measurements allow predictions of regulation and metabolic behavior in Methanosarcina acetivorans [PDF]
Metabolic Model and Maps. A zip file archive containing the iST807 metabolic model in the SBML format, an Excel spreadsheet including descriptions of the metabolic model and the metabolic map in formats compatible with Cytoscape and Escher.
Joseph R. Peterson +5 more
core +6 more sources
The redox regulation of proteins via reversible dithiol/disulfide exchange reactions involves the thioredoxin system, which is composed of a reductant, a thioredoxin reductase (TR), and thioredoxin (Trx).
Rubén M. Buey +3 more
doaj +1 more source
Genetic encoding of noncanonical amino acids (ncAAs) through sense codon reassignment is an efficient tool for expanding the chemical functionality of proteins. Incorporation of multiple ncAAs, however, is particularly challenging.
Alessandro De Simone +3 more
doaj +1 more source

