Enhancing the Sensitivity of Mass Spectrometry Imaging through Spatial Signal Averaging. [PDF]
Desyaterik YN +8 more
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Robustness of Protein-Ligand Binding Affinity Prediction Models to Docked and Predicted Structures. [PDF]
Eaves JN, Woldring DR.
europepmc +1 more source
Heterotropic regulation and negative homotropic cooperativity
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
Beyond Directions: Symmetry-Aware Rotation Sets for Triaxial Diffusion Encoding by Geometric Filter Optimization. [PDF]
Jespersen SN, Szczepankiewicz F.
europepmc +1 more source
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta +7 more
wiley +1 more source
FedMCP++: Integrating Modular Expert Heads with Prototype-Guided Contrastive Distillation for Wireless Personalized Federated Learning. [PDF]
Günay FB, Bozkurt F.
europepmc +1 more source
Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer +7 more
wiley +1 more source
Multi-fidelity Monte Carlo fusion for BNCT dose calculation using a dual-branch network. [PDF]
Li L +7 more
europepmc +1 more source

