Results 81 to 90 of about 241,169 (264)

Causes and methods of overcoming of the contradictions in law positivistic views (legism and sociology)

RUDN Journal of Law, 2014
The article describes the history of occurrence of positivism as a scientific course, its main ideas, the nature of the positivist school, as well as the problem of the causes and ways to overcome collisions in the law in modern legal literature, mainly ...
K V Akhmetzhanova
doaj  

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Directed evolution methods for overcoming trade-offs between protein activity and stability. [PDF]

AIChE J, 2020
Stimple SD, Smith MD, Tessier PM.
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

A mixed-methods observational study of strategies for success in implementation science: overcoming emergency departments hurdles

BMC Health Services Research
Background Heart failure is a major public health concern, affecting 6.7 million Americans. An estimated 16% of emergency department (ED) patients with acute heart failure (AHF) are discharged home.
Deonni P. Stolldorf, Alan B. Storrow, Dandan Liu, Cathy A. Jenkins, Rachel A. Hilton, Karen F. Miller, Joy Kim, Deepika Boopathy, Satheesh Gunaga, Bory Kea, Joseph Miller, Sean P. Collins   +11 more
doaj   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Fosfomycin—Overcoming Problematic In Vitro Susceptibility Testing and Tricky Result Interpretation: Comparison of Three Fosfomycin Susceptibility Testing Methods

Antibiotics
Background: Fosfomycin (FOS) is an older antimicrobial agent newly rediscovered as a possible treatment for infections with limited therapeutic options (e.g., Gram-negative bacteria with difficult-to-treat resistance, DTR), especially in intravenous form.
Jan Závora, Gabriela Kroneislová, Marie Kroneisl, Václava Adámková   +3 more
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

ESTIMATE OF EFFICIENCY OF ENEMY AIR DEFENCE OVERCOMING BY AIRCRAFT

Sučasnì Informacìjnì Tehnologìï u Sferì Bezpeki ta Oboroni, 2013
In this article the methods of estimate of efficiency of an air defence system overcoming by one and a group of airplanes and also a general estimation efficiency overcoming of an air defence system are examined.
Spartak Y. Hohoniants, Oleksii A. Uleshchenko   +1 more
doaj  

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source