Results 41 to 50 of about 1,947,841 (302)

Human Apolipoprotein B Transgenic Mice Generated with 207- and 145-Kilobase Pair Bacterial Artificial Chromosomes. Evidence that a distant 5'-element confers appropriate transgene expression in the intestine [PDF]

, 1997
We reported previously that ~80-kilobase pair (kb) P1 bacteriophage clones spanning either the human or mouse apoB gene (clones p158 and p649, respectively) confer apoB expression in the liver of transgenic mice, but not in the intestine. We hypothesized
Gunn, Michael D., McCormick, Sally P. A., Nielsen, Lars B., Pierotti, Vincenzo, Shizuya, Hiroaki, Tam, Carmen, Young, Stephen G.   +6 more
core  

Prospects for transgenesis in the chick [PDF]

, 2004
Research to develop a useful method for genetic modification of the chick has been on-going since the first demonstrations in the mouse in the 1980s that genetic modification is an invaluable tool for the study of gene function. Manipulation of the chick
Bosselman, Brazolot, Carsience, Challita, D'Costa, Evans, Eyal-Giladi, Hamburger, Han, Harvey, Harvey, Hofmann, Ikawa, Isvak, Itasaki, Ivarie, Kagami, Lois, Love, Mizuarai, Mozdziak, Mozdziak, Naito, Naito, Naito, Naldini, Nieuwkoop, Niwa, Pain, Perry, Petitte, Petitte, Pfeifer, Rapp, Reynaud, Rubinson, Salter, Salter, Sang, Sherman, Speksnijder, Stern, Tajima, Thomson, Thoraval, Tiscornia, Tsunekawa, Urven, Vick, Vick, Waddington, Wentworth, Zajchowski   +52 more
core   +1 more source

Production of transgenic mice

Folia Pharmacologica Japonica, 1994
Transgenic mice are very useful for analyzing the functions of a gene either at the tissue level or at the whole body level. Here we describe the production of transgenic mice by the microinjection of DNA into the pronuclei of fertilized mouse eggs.
openaire   +5 more sources

Retinoblastoma in transgenic mice

Nature, 1990
Retinoblastoma, a malignancy of the eye occurring in young children, has been widely studied as a model for genetic predisposition to cancer. This disease is caused by mutations in both alleles of an anti-oncogene (the retinoblastoma gene, Rb) that inactivate or eliminate the Rb encoded protein, p105Rb (refs 1 and 2).
Windle, J.J., Albert, D.M., O'Brien, J.M., Marcus, D.M., Disteche, Ch.M., Bernards, R.A., Mellon, P.L.   +6 more
openaire   +3 more sources

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Function and mechanism of the human SOD2 gene in mice cerebral ischemia/ reperfusion injury [PDF]

Acta Cirúrgica Brasileira
Purpose: To investigate the neuroprotective effects of the SOD2 gene in cerebral ischemia reperfusion injury function and the underlying mechanisms in a mice model of middle cerebral artery ischemia reperfusion.
Xitong Yang, Guangming Wang
doaj   +1 more source

Reduced antioxidant enzyme activity in brains of mice transgenic for human presenilin-1 with single or multiple mutations [PDF]

, 2006
Alzheimer's disease-related mutations in the presenilin-1 gene (PS1) are leading to an elevated production of neurotoxic beta-amyloid 1-42 and may additionally enhance oxidative stress.
Czech, Christian, Eckert, Anne, Leutner, Silke, Müller, Walter E., Schindowski, Katharina, Touchet, Nathalie   +5 more
core  

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Enhanced UV-Induced Skin Carcinogenesis in Transgenic Mice Overexpressing Proprotein Convertases

Neoplasia: An International Journal for Oncology Research, 2013
The proprotein convertases (PCs) furin and PACE4 process numerous substrates involved in tumor growth, invasion, and metastasis. We have previously shown that PCs increase the susceptibility to chemical skin carcinogenesis. Because of the human relevancy
Jian Fu, Daniel E. Bassi, Jirong Zhang, Tianyu Li, Kathy Q. Cai, Courtney Lyons Testa, Emmanuelle Nicolas, Andres J. Klein-Szanto   +7 more
doaj   +1 more source

Colorectal cancer‐derived FGF19 is a metabolically active serum biomarker that exerts enteroendocrine effects on mouse liver

Molecular Oncology, EarlyView.
Meta‐transcriptome analysis identified FGF19 as a peptide enteroendocrine hormone associated with colorectal cancer prognosis. In vivo xenograft models showed release of FGF19 into the blood at levels that correlated with tumor volumes. Tumoral‐FGF19 altered murine liver metabolism through FGFR4, thereby reducing bile acid synthesis and increasing ...
Jordan M. Beardsley, Michael W. Rohr, Joseph A. Goode, Sai Preethi Nakkina, Jignesh G. Parikh, Deborah A. Altomare   +5 more
wiley   +1 more source