Results 51 to 60 of about 280,806 (266)

Enzymatic degradation of biopolymers in amorphous and molten states: mechanisms and applications

FEBS Open Bio, EarlyView.
This review explains how polymer morphology and thermal state shape enzymatic degradation pathways, comparing amorphous and molten biopolymer structures. By integrating structure–reactivity principles with insights from thermodynamics and enzyme engineering, it highlights mechanisms that enable efficient polymer breakdown.
Anđela Pustak, Aleksandra Maršavelski
wiley   +1 more source

The engineering of TBBPA-degrading synthetic microbiomes with integrated strategies

npj Biofilms and Microbiomes
The capability to understand and construct synthetic microbiomes is crucial in biotechnological innovation and application. Tetrabromobisphenol A (TBBPA) is an emerging pollutant, and the understanding of its biodegradation is very limited.
Tong Wu, Sheng-Zhi Guo, Yi Zhang, Xi-Ze Zhao, Cong-Guo Ran, Feng-Lan Liu, Run-Hua Wang, De-Feng Li, Hai-Zhen Zhu, Cheng-Ying Jiang, Xi-Hui Shen, Shuang-Jiang Liu   +11 more
doaj   +1 more source

Tumor‐stromal crosstalk and macrophage enrichment are associated with chemotherapy response in bladder cancer

FEBS Open Bio, EarlyView.
Chemoresistance in bladder cancer: Macrophage recruitment associated with CXCL1, CXCL5 and CXCL8 expression is characteristic of Gemcitabine/Cisplatin (Gem/Cis) Non‐Responder tumors (right side) while Responder tumors did not show substantial tumor‐stromal crosstalk (left side). All biological icons are attributed to Bioicons: carcinoma, cancerous‐cell‐
Sophie Leypold, Janik Riese, Lancelot Seillier, Mark Kühnel, Julia Pannhausen, Charlotte O. J. Fröhlich, Christian Martin, Peter Boor, Matthias Saar, Danny D. Jonigk, Nadine T. Gaisa, Michael Rose   +11 more
wiley   +1 more source

Understanding bio‐based polymers: A study of origins, properties, biodegradation and their impact on health and the environment

FEBS Open Bio, EarlyView.
This review provides an overview of bio‐based polymer sources, their unique functional properties and their environmental impact, and addresses their role as sustainable alternatives. It discusses end‐of‐life options, including composting and anaerobic digestion for renewable energy.
Sabina Kolbl Repinc, Blaž Stres, Mirica Karlovits, Igor Karlovits, Petra Jerič, Ondrej Panák, Anja Verbič, Blaž Likozar, Uroš Novak   +8 more
wiley   +1 more source

A Unique THN Motif Is Critical for Enabling Efficient C‐Terminal Traceless Cleavage

Advanced Science
Traceless protein cleavage is a significant challenge in intein application, as most common inteins studied today are not both active and promiscuous.
Ruocheng Gu, Yunuo Lin, Rouyu Di, Tongtong Zhou, Tingwen Fan, Wei Li, Lili Miao, Huaiyi Yang   +7 more
doaj   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Engineering Methylotrophic Yeasts as Cell Factories for Chemical Production Using Methanol as a Feedstock

GCB Bioenergy
Methanol, a sustainable and abundant one‐carbon (C1) feedstock, has emerged as a promising raw material for green biomanufacturing, offering a pathway to carbon neutrality. Natural methylotrophic yeasts such as Pichia pastoris (syn. Komagataella phaffii)
Shuxian Wang, Jiayu Fang, Yanping Zhang, Yin Li, Taicheng Zhu   +4 more
doaj   +1 more source

Mining Microbial Dark Matter: Advanced Cultivation Techniques for Bioactive Compound Discovery

Pharmaceuticals
The vast majority of microorganisms in the environment remain uncultured using conventional laboratory techniques, representing an immense untapped reservoir of genetic and chemical diversity.
Minhui Ji, Bingda Ma, Jiayu Dong, Shan Liu, Ying Shi, Meiting Bu, Luoyi Wang, Ling Liu   +7 more
doaj   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

What is Fusarium forma specialis?

Mycology
The forma specialis concept has been a cornerstone in Fusarium phytopathology for 85 years, classifying pathogens based on host specificity. However, its validity as a natural and practical framework has been increasingly questioned.
Shiling Han, Peng Zhao, Mengmeng Wang, Xiaoyu Jiang, Fang Liu, Junen Huang, Lei Cai   +6 more
doaj   +1 more source