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Metagenomic discovery of microbial eukaryotes in stool microbiomes [PDF]

open access: yesmBio
Host-associated microbiota form complex microbial communities that are increasingly associated with host behavior and disease. While these microbes include bacterial, archaeal, viral, and eukaryotic constituents, most studies have focused on bacteria due
Audra L. Crouch   +5 more
doaj   +4 more sources

Missing microbial eukaryotes and misleading meta-omic conclusions [PDF]

open access: yesNature Communications, 2023
Meta-omics is commonly used for large-scale analyses of microbial eukaryotes, including species or taxonomic group distribution mapping, gene catalog construction, and inference on the functional roles and activities of microbial eukaryotes in situ. Here,
Arianna I. Krinos   +7 more
doaj   +6 more sources

Microbial Eukaryotes Associated With Sediments in Deep-Sea Cold Seeps [PDF]

open access: yesFrontiers in Microbiology, 2021
Microbial eukaryotes are key components of the marine food web, but their distribution in deep-sea chemosynthetic ecosystems has not been well studied. Here, high-throughput sequencing of the 18S rRNA gene and network analysis were applied to investigate
Yue Zhang   +8 more
doaj   +2 more sources

Accurate and sensitive detection of microbial eukaryotes from whole metagenome shotgun sequencing [PDF]

open access: yesMicrobiome, 2021
Background Microbial eukaryotes are found alongside bacteria and archaea in natural microbial systems, including host-associated microbiomes. While microbial eukaryotes are critical to these communities, they are challenging to study with shotgun ...
Abigail L. Lind, Katherine S. Pollard
doaj   +2 more sources

DNA extraction bias is more pronounced for microbial eukaryotes than for prokaryotes [PDF]

open access: yesMicrobiologyOpen, 2022
DNA extraction and preservation bias is a recurring topic in DNA sequencing‐based microbial ecology. The different methodologies can lead to distinct outcomes, which has been demonstrated especially in studies investigating prokaryotic community ...
Anne Brauer, Mia M. Bengtsson
doaj   +2 more sources

A single-cell genomics pipeline for environmental microbial eukaryotes [PDF]

open access: yesiScience, 2021
Summary: Single-cell sequencing of environmental microorganisms is an essential component of the microbial ecology toolkit. However, large-scale targeted single-cell sequencing for the whole-genome recovery of uncultivated eukaryotes is lagging.
Doina Ciobanu   +21 more
doaj   +2 more sources

Genetic Diversity, Community Assembly, and Shaping Factors of Benthic Microbial Eukaryotes in Dongshan Bay, Southeast China [PDF]

open access: yesFrontiers in Microbiology, 2020
Microbial eukaryotes are pivotal components of marine ecosystems. However, compared with the pelagic environments, the diversity distribution and the driving mechanisms of microbial eukaryotes in the marine sediments have rarely been explored.
Rong Gu   +14 more
doaj   +2 more sources

Molecular architecture of giant viruses infecting microbial eukaryotes (protists) [PDF]

open access: yesBioTechnologia
In this review, I describe recent findings on the molecular architecture and genomic characterization of giant viruses that infect microbial eukaryotes (protists) across diverse ecosystems and ecological niches.
Anhelina Kyrychenko
doaj   +2 more sources

An efficient single-cell transcriptomics workflow for microbial eukaryotes benchmarked on Giardia intestinalis cells [PDF]

open access: yesBMC Genomics, 2020
Background Most diversity in the eukaryotic tree of life is represented by microbial eukaryotes, which is a polyphyletic group also referred to as protists.
Henning Onsbring   +4 more
doaj   +2 more sources

Microbial Eukaryotes: a Missing Link in Gut Microbiome Studies [PDF]

open access: yesmSystems, 2018
Human-associated microbial communities include prokaryotic and eukaryotic organisms across high-level clades of the tree of life. While advances in high-throughput sequencing technology allow for the study of diverse lineages, the vast majority of ...
Isabelle Laforest-Lapointe   +1 more
doaj   +2 more sources

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