Results 91 to 100 of about 728,080 (310)

New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica

Microbial Cell Factories, 2017
Background The oleaginous yeast Yarrowia lipolytica is increasingly used as alternative cell factory for the production of recombinant proteins. At present, several promoters with different strengths have been developed based either on the constitutive ...
Marion Trassaert, Marie Vandermies, Fréderic Carly, Olivia Denies, Stéphane Thomas, Patrick Fickers, Jean-Marc Nicaud   +6 more
doaj   +1 more source

Minimal Interspecies Interaction Adjustment (MIIA): Inference of Neighbor-Dependent Interactions in Microbial Communities [PDF]

, 2019
An intriguing aspect in microbial communities is that pairwise interactions can be influenced by neighboring species. This creates context dependencies for microbial interactions that are based on the functional composition of the community.
Bernstein, Hans C., Fredrickson, Jim K., Haruta, Shin, Lee, Dong-Yup, Lee, Joon-Yong, Lindemann, Stephen R., Nelson, William C., Song, Hyun-Seob   +7 more
core   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Efficient expression vectors and host strain for the production of recombinant proteins by Yarrowia lipolytica in process conditions

Microbial Cell Factories, 2019
Background The oleaginous yeast Yarrowia lipolytica is increasingly used as an alternative cell factory for the production of recombinant proteins. Recently, regulated promoters from genes EYK1 and EYD1, encoding an erythrulose kinase and an erythritol ...
Young-Kyoung Park, Marie Vandermies, Paul Soudier, Samuel Telek, Stéphane Thomas, Jean-Marc Nicaud, Patrick Fickers   +6 more
doaj   +1 more source

Raman Spectroscopy-Based Measurements of Single-Cell Phenotypic Diversity in Microbial Populations

mSphere, 2020
Microbial cells experience physiological changes due to environmental change, such as pH and temperature, the release of bactericidal agents, or nutrient limitation.
Cristina García-Timermans, Ruben Props, Boris Zacchetti, Myrsini Sakarika, Frank Delvigne, Nico Boon   +5 more
doaj   +1 more source

Plant and soil microbe responses to light, warming and nitrogen addition in a temperate forest [PDF]

, 2018
1. Temperate forests across Europe and eastern North America have become denser since the 1950s due to less intensive forest management and global environmental changes such as nitrogen deposition and climate warming. Denser tree canopies result in lower
Boeckx, Pascal, Boon, Nico, De Frenne, Pieter, Ma, Shiyu, Props, Ruben, Vanhellemont, Margot, Verheyen, Kris, Wasof, Safaa   +7 more
core   +2 more sources

Natural Products as Geroprotective Modulators in Diabetic Nephropathy: A Mechanistic Framework Integrating Aging Hallmarks and the AMPK–SIRT1–Nrf2 Axis

Aging and Cancer, EarlyView.
Natural products target the aging kidney in diabetic nephropathy by restoring the AMPK–SIRT1–Nrf2 axis, reducing oxidative stress, inflammation, fibrosis, and cellular senescence while enhancing mitochondrial biogenesis and antioxidant defenses.
Sherif Hamidu, Latif Adams, Eunice Ampem‐Danso, Justice Kumi, Abigail Aning, Fatima Hamidu, Mustapha Abdul Mumin Mohammed, Ayuba Nii Darku Ankrah, Seth Kwabena Amponsah   +8 more
wiley   +1 more source

Agricultural management and plant selection interactively affect rhizosphere microbial community structure and nitrogen cycling. [PDF]

, 2019
BACKGROUND:Rhizosphere microbial communities are key regulators of plant performance, yet few studies have assessed the impact of different management approaches on the rhizosphere microbiomes of major crops.
Brisson, Vanessa L, Gaudin, Amélie CM, Kent, Angela D, Schmidt, Jennifer E   +3 more
core   +1 more source

Advancing Research on Biomaterials and Biological Materials with Scanning Electron Microscopy under Environmental and Low Vacuum Conditions

Advanced Engineering Materials, EarlyView.
Herein, environmental scanning electron microscopy (ESEM) is discussed as a powerful extension of conventional SEM for life sciences. By combining high‐resolution imaging with variable pressure and humidity, ESEM allows the analysis of untreated biological materials, supports in situ monitoring of hydration‐driven changes, and advances the functional ...
Jendrian Riedel, Lea Dierker, Antoine Eyram Kwame, Kim Nora Wagner, Jonas J. Unterholzner, Jan‐Henning Dirks, Dorothea Brüggemann   +6 more
wiley   +1 more source

Template assisted surface micro microstructuring of flowable dental composites and its effect on the microbial adhesion properties [PDF]

, 2016
Despite their various advantages, such as good esthetic properties, absence of mercury and adhesive bonding to teeth, modern dental composites still have some drawbacks, e.g., a relatively high rate of secondary caries on teeth filled with composite ...
Frenzel, Nadja, Jandt, Klaus D., Lüdecke, Claudia, Maenz, Stefan, Sanz Beltrán, Vanesa, Sigusch, Bernd W., Völpel, Andrea   +6 more
core   +1 more source