Results 191 to 200 of about 47,561 (246)

Proteomics and Laser Microdissection

2006
Two-dimensional gel electrophoresis (2-DE) combined with protein identification by mass spectrometry (MS) is currently the method of choice in the majority of proteomic projects. Novel gel-free technologies have been developed but 2-DE remains the technique of choice for quantitative expression profiling of large sets of complex protein mixtures such ...
Emma, McGregor, Ayesha, De Souza
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Microdissection testicular sperm extraction (micro-TESE) in men with infertility due to nonobstructive azoospermia: summary of current literature

International Urology and Nephrology, 2021
A. Achermann, Thairo A Pereira, S. Esteves   +2 more
semanticscholar   +1 more source

Microdissection and the Study of Cancer Pathways

Current Molecular Medicine, 2001
Abstract: The study of genetic alterations in tumors and their precursor lesions is often hampered by the presence of a heterogeneous background of non-neoplastic elements such as stromal cells, inflammatory cells, and angiogenic elements. Microdissection involves the extraction of specific populations of cells under direct visualization.
A, Maitra, I I, Wistuba, A F, Gazdar
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Manual Microdissection

2011
No abstract ...
FAORO, VALENTINA, STANTA, GIORGIO
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Microdissection and microcloning of plant chromosomes

Cytogenetic and Genome Research, 2005
Cytogenetic and molecular tools play an increasingly important role in plant genome research. A number of interesting applications that involve chromosome painting, the relationship between specific chromosomes and specific linkage groups, the relationships between physical and genetic distances on linkage maps, and the isolation of genes of interest ...
A, Fominaya, C, Linares, Y, Loarce, E, Ferrer   +3 more
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Reliable detection of somatic mutations in solid tissues by laser-capture microdissection and low-input DNA sequencing

Nature Protocols, 2020
Peter Ellis, L. Moore, M. Sanders, T. Butler, Simon F. Brunner, H. Lee-Six, Robert J. Osborne, Ben W. Farr, Tim H. H. Coorens, A. Lawson, A. Cagan, M. Stratton, I. Martincorena, P. Campbell   +13 more
semanticscholar   +1 more source

Microdissection of Histologic Sections: Manual and Laser Capture Microdissection Techniques

2003
The molecular analysis of human cancer is complicated by the difficulty in obtaining pure populations of tumor cells to study. One traditional method of obtaining a pure representation has been establishing cancer cell lines from primary tumors. However, this technique is time consuming and of low yield.
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V.—ELECTRON MIOROSCOPY AND MICRODISSECTION

Journal of the Royal Microscopical Society, 1952
A new technique is described for the preparation of biological specimens for electron microscope study, a de Fonbrune micromanipulator and an inverted microscope being used. The microdissection is made directly on the collodion film supported by a thin glass slide (cover‐slip) and afterwards the specimen is mounted on a grid.
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[Microdissection of chromosomes].

Ugeskrift for laeger, 2000
The technique of deciphering different chromosome abnormalities by the employment of specific DNA probes prepared by microdissection of normal or abnormal chromosomes is described. It is emphasised that in some families the diagnosis is possible only by "family specific" probes.
U K, Friedrich, M, Houman
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Chromosome microtechnology: microdissection and microcloning

Trends in Biotechnology, 1992
The physical microdissection of chromosomes and subsequent microcloning of dissected fragments is enabling the generation of very large numbers of cloned unique sequences from defined chromosomal regions. In addition to use in constructing region-specific libraries of the entire human genome and providing probes for mapping and sequencing purposes ...
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