Results 181 to 190 of about 47,561 (246)
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Microdissection of Mouse Brain into Functionally and Anatomically Different Regions.
Journal of Visualized Experiments, 2021The brain is the command center for the mammalian nervous system and an organ with enormous structural complexity. Protected within the skull, the brain consists of an outer covering of grey matter over the hemispheres known as the cerebral cortex ...
J. Meyerhoff +7 more
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Microdissection of the Rodent Eye
Journal of Visualized Experiments, 2023The ocular micro-dissection of the rodent eye involves the segmentation of the enucleated eyeball with the attached nictitating membrane, or third eyelid, to obtain the anterior and posterior eyecups. With this technique, the sub-parts of the eye, including the corneal tissue, neural tissue, retinal pigment epithelial (RPE) tissue, and lens, can be ...
K Varsha, Mohan +5 more
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Current Protocols in Human Genetics, 1997
AbstractThis unit describes chromosome microdissection methodologies that are useful in both clinical and research laboratories. In combination with FISH, region‐specific probes can be made to determine the origin of marker chromosomes. Another application of the microdissection technology is in the generation of regionspecific cDNA libraries, known as
P, Meltzer, M, Bittner
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AbstractThis unit describes chromosome microdissection methodologies that are useful in both clinical and research laboratories. In combination with FISH, region‐specific probes can be made to determine the origin of marker chromosomes. Another application of the microdissection technology is in the generation of regionspecific cDNA libraries, known as
P, Meltzer, M, Bittner
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PROTEOMICS, 2007
Abstract The complexity of the proteome is extremely high, because every organ or even a part of it can differ considerably in its protein composition. Performing proteomic studies therefore means to separate these functional different tissue areas before analysis. Otherwise all gained results will be depending on the question whether
Ferdinand, von Eggeling +2 more
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Abstract The complexity of the proteome is extremely high, because every organ or even a part of it can differ considerably in its protein composition. Performing proteomic studies therefore means to separate these functional different tissue areas before analysis. Otherwise all gained results will be depending on the question whether
Ferdinand, von Eggeling +2 more
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2008
Procurement of pure populations of cells from heterogeneous histological sections can be accomplished utilizing tissue microdissection. At present, a variety of different manual and laser-based dissection tools are available and each method has particular strengths and weaknesses.
Heidi S, Erickson +2 more
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Procurement of pure populations of cells from heterogeneous histological sections can be accomplished utilizing tissue microdissection. At present, a variety of different manual and laser-based dissection tools are available and each method has particular strengths and weaknesses.
Heidi S, Erickson +2 more
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Microdissection of the mouse egg
Experimental Cell Research, 1983A rapid and efficient method for microdissection of the mouse egg is described. The dissection is carried out in hanging drops of medium surrounded by heavy liquid paraffin oil at room temperature. Eggs are first deformed into a cylindrical shape and then dissected at a predetermined site with a glass needle on a Leitz micromanipulator.
S C, Barton, M A, Surani
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2009
The new opportunities of modern assays of molecular biology can only be exploited fully if the results can be accurately correlated to the tissue phenotype under investigation. This is a general problem of non-in situ techniques, whereas results from in situ techniques are often difficult to quantitate.
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The new opportunities of modern assays of molecular biology can only be exploited fully if the results can be accurately correlated to the tissue phenotype under investigation. This is a general problem of non-in situ techniques, whereas results from in situ techniques are often difficult to quantitate.
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Tissue Microdissection and Processing
2001The accurate analysis of molecular changes associated with tumors and their precursor lesions requires the precise isolation of the specific cell types from a heterogeneous background of non-neoplastic elements such as normal epithelium, desmoplastic stroma, inflammatory cells and blood vessels(1).In the absence of a prior cell enrichment technique ...
A, Maitra, A F, Gazdar
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2009
Gene expression analysis requires a sound basis of cell material to obtain realistic results. Tissue, however, consists of diverse types of cells, which often differentially express target genes, so that cell populations need to be selected. If tissue diversity is moderate and negligible, manual microdissection can be the cost-efficient method of ...
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Gene expression analysis requires a sound basis of cell material to obtain realistic results. Tissue, however, consists of diverse types of cells, which often differentially express target genes, so that cell populations need to be selected. If tissue diversity is moderate and negligible, manual microdissection can be the cost-efficient method of ...
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Laser capture microdissection: Big data from small samples
Histology and Histopathology, 2015Any tissue is made up of a heterogeneous mix of spatially distributed cell types. In response to any (patho) physiological cue, responses of each cell type in any given tissue may be unique and cannot be homogenized across cell-types and spatial co ...
Soma Datta +5 more
semanticscholar +1 more source