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Current Protocols in Molecular Biology, 2015
AbstractLaser microdissection (LM) offers a relatively rapid and precise method of isolating and removing specified cells from complex tissues for subsequent analysis of their RNA, DNA, protein or metabolite content, thereby allowing assessment of the role of different cell types in the normal physiological or disease processes being studied.
Andra R, Frost +4 more
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AbstractLaser microdissection (LM) offers a relatively rapid and precise method of isolating and removing specified cells from complex tissues for subsequent analysis of their RNA, DNA, protein or metabolite content, thereby allowing assessment of the role of different cell types in the normal physiological or disease processes being studied.
Andra R, Frost +4 more
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PROTEOMICS, 2007
Abstract The complexity of the proteome is extremely high, because every organ or even a part of it can differ considerably in its protein composition. Performing proteomic studies therefore means to separate these functional different tissue areas before analysis. Otherwise all gained results will be depending on the question whether
Ferdinand, von Eggeling +2 more
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Abstract The complexity of the proteome is extremely high, because every organ or even a part of it can differ considerably in its protein composition. Performing proteomic studies therefore means to separate these functional different tissue areas before analysis. Otherwise all gained results will be depending on the question whether
Ferdinand, von Eggeling +2 more
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2016
The new opportunities of modern assays of molecular biology can only be exploited fully if the results can be accurately correlated to the tissue phenotype under investigation. This is a general problem of non-in situ techniques, whereas results from in situ techniques are often difficult to quantify.
Anja, Rabien, Glen, Kristiansen
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The new opportunities of modern assays of molecular biology can only be exploited fully if the results can be accurately correlated to the tissue phenotype under investigation. This is a general problem of non-in situ techniques, whereas results from in situ techniques are often difficult to quantify.
Anja, Rabien, Glen, Kristiansen
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Science, 1996
Laser capture microdissection (LCM) under direct microscopic visualization permits rapid one-step procurement of selected human cell populations from a section of complex, heterogeneous tissue. In this technique, a transparent thermoplastic film (ethylene vinyl acetate polymer) is applied to the surface of the tissue section on a standard glass ...
M R, Emmert-Buck +7 more
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Laser capture microdissection (LCM) under direct microscopic visualization permits rapid one-step procurement of selected human cell populations from a section of complex, heterogeneous tissue. In this technique, a transparent thermoplastic film (ethylene vinyl acetate polymer) is applied to the surface of the tissue section on a standard glass ...
M R, Emmert-Buck +7 more
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Microdissection of the mouse egg
Experimental Cell Research, 1983A rapid and efficient method for microdissection of the mouse egg is described. The dissection is carried out in hanging drops of medium surrounded by heavy liquid paraffin oil at room temperature. Eggs are first deformed into a cylindrical shape and then dissected at a predetermined site with a glass needle on a Leitz micromanipulator.
S C, Barton, M A, Surani
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Tissue Microdissection and Processing
2001The accurate analysis of molecular changes associated with tumors and their precursor lesions requires the precise isolation of the specific cell types from a heterogeneous background of non-neoplastic elements such as normal epithelium, desmoplastic stroma, inflammatory cells and blood vessels(1).In the absence of a prior cell enrichment technique ...
A, Maitra, A F, Gazdar
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2009
Gene expression analysis requires a sound basis of cell material to obtain realistic results. Tissue, however, consists of diverse types of cells, which often differentially express target genes, so that cell populations need to be selected. If tissue diversity is moderate and negligible, manual microdissection can be the cost-efficient method of ...
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Gene expression analysis requires a sound basis of cell material to obtain realistic results. Tissue, however, consists of diverse types of cells, which often differentially express target genes, so that cell populations need to be selected. If tissue diversity is moderate and negligible, manual microdissection can be the cost-efficient method of ...
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Proteomics and Laser Microdissection
2006Two-dimensional gel electrophoresis (2-DE) combined with protein identification by mass spectrometry (MS) is currently the method of choice in the majority of proteomic projects. Novel gel-free technologies have been developed but 2-DE remains the technique of choice for quantitative expression profiling of large sets of complex protein mixtures such ...
Emma, McGregor, Ayesha, De Souza
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Microdissection of Histologic Sections: Manual and Laser Capture Microdissection Techniques
2003The molecular analysis of human cancer is complicated by the difficulty in obtaining pure populations of tumor cells to study. One traditional method of obtaining a pure representation has been establishing cancer cell lines from primary tumors. However, this technique is time consuming and of low yield.
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