Results 41 to 50 of about 97,479 (268)

Loss of proton‐sensing TDAG8 increases tumor progression in mouse models of colon cancer

Molecular Oncology, EarlyView.
Loss of the pH‐sensing receptor TDAG8 accelerates colorectal cancer progression in mice. Animals lacking TDAG8 expression had increased tumor growth, DNA damage, and recruitment of tumor‐associated immune cells, including macrophages, neutrophils, and monocytes.
Ermanno Malagola, Yasmine Illi, Anna Bircher, Marijn Wilmink, Rachele F. Malagutti, Solenn Ottié, Cordelia Schuler, Pedro A. Ruiz, Cheryl de Vallière, Klaus Seuwen, Gerhard Rogler, Martin Hausmann   +11 more
wiley   +1 more source

Microfluidics in biotechnology [PDF]

Journal of Nanobiotechnology, 2004
Microfluidics enables biotechnological processes to proceed on a scale (microns) at which physical processes such as osmotic movement, electrophoretic-motility and surface interactions become enhanced. At the microscale sample volumes and assay times are reduced, and procedural costs are lowered.
Barry, Richard, Ivanov, Dimitri
openaire   +4 more sources

Spatial biology in cancer epigenetics

Molecular Oncology, EarlyView.
Spatial epigenomics combines molecular profiling with tissue architecture to reveal how gene regulation is organized within intact tissues. In cancer, these technologies uncover the mechanisms driving tumor heterogeneity and microenvironmental interactions, opening new opportunities for biomarker discovery and precision medicine.
Eva Crespo‐García, Manel Esteller
wiley   +1 more source

Construction of a Highly Stable Water-Based Release Agent via 1:1 Silicone Oil-Cyclotetrasiloxane Synergy

Molecules
This study develops a high-performance water-based mold release agent for polyurethane (PU) foaming applications. We demonstrate that incorporating octamethylcyclotetrasiloxane (D4) into a dimethyl silicone oil emulsion (5 vol% fixed concentration ...
Can Wang, Yutong Han, Xiaojuan Du, Sihan Guo, Qiming Zhao, Xiao Chen   +5 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

A Real-Time Centrifugal Microfluidic Chip with Dual-Valving Strategy for Multiplexed PCR Detection at Point-of-Care Testing

Chemosensors
Different from isothermal amplification, for polymerase chain reaction (PCR), highly reliable valving for PCR chamber, significantly shortened thermal cycling time, and concise multiplexed detection are always challenges for microfluidic-based devices ...
Yize Zhang, Youhong Zeng, Lingxuan Liu, Lei Wang, Hao Chen, Yatan Yuan, Yingying Ding, Guijun Miao, Lulu Zhang, Xianbo Qiu   +9 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

A Low-Cost Handheld Centrifugal Microfluidic System for Multiplexed Visual Detection Based on Isothermal Amplification

Sensors
A low-cost, handheld centrifugal microfluidic system for multiplexed visual detection based on recombinase polymerase amplification (RPA) was developed.
Nan Wang, Xiaobin Dong, Yijie Zhou, Rui Zhu, Luyao Liu, Lulu Zhang, Xianbo Qiu   +6 more
doaj   +1 more source

Cutting-Edge Technologies for Inflamed Joints on Chip: How Close Are We?

Frontiers in Immunology, 2022
Osteoarthritis (OA) is a painful and disabling musculoskeletal disorder, with a large impact on the global population, resulting in several limitations on daily activities.
Emine Kahraman, Emine Kahraman, Emine Kahraman, Ricardo Ribeiro, Ricardo Ribeiro, Meriem Lamghari, Meriem Lamghari, Estrela Neto, Estrela Neto   +8 more
doaj   +1 more source

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source