Results 41 to 50 of about 123,453 (319)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Mechanical Properties of Stainless-Steel Structures Fabricated by Composite Extrusion Modelling

Metals, 2018
Composite Extrusion Modelling (CEM) is a new additive manufacturing process for metal that uses Metal Injection Moulding (MIM) materials. The MIM material is printed on a build plate using a moveable extruder.
Clemens Lieberwirth, Mohamed Sarhan, Hermann Seitz   +2 more
doaj   +1 more source

Paper-Based Bi-Material Cantilever Actuator Bending Behavior and Modeling

Micromachines, 2023
In this paper, the behavior of the Bi-Material Cantilever (B-MaC) response deflection upon fluidic loading was experimentally studied and modeled for bilayer strips. A B-MaC consists of a strip of paper adhered to a strip of tape.
Gordon Chen, Ashutosh Kumar, Hojat Heidari-Bafroui, Winfield Smith, Amer Charbaji, Nassim Rahmani, Constantine Anagnostopoulos, Mohammad Faghri   +7 more
doaj   +1 more source

Microfluidic sterilization [PDF]

Biomicrofluidics, 2014
Nowadays, microfluidics is attracting more and more attentions in the biological society and has provided powerful solutions for various applications. This paper reported a microfluidic strategy for aqueous sample sterilization. A well-designed small microchannel with a high hydrodynamic resistance was used to function as an in-chip pressure regulator.
Rui, Zhang, Jie, Huang, Fei, Xie, Baojun, Wang, Ming, Chu, Yuedan, Wang, Haichao, Li, Wei, Wang, Haixia, Zhang, Wengang, Wu, Zhihong, Li   +10 more
openaire   +2 more sources

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Cell Patterning Technology on Polymethyl Methacrylate through Controlled Physicochemical and Biochemical Functionalization

Biosensors, 2023
In recent years, innovative cell-based biosensing systems have been developed, showing impact in healthcare and life science research. Now, there is a need to design mass-production processes to enable their commercialization and reach society.
Enrique Azuaje-Hualde, Job Komen, Juncal A. Alonso-Cabrera, Albert van den Berg, Marian M. de Pancorbo, Andries D. van der Meer, Fernando Benito-Lopez, Lourdes Basabe-Desmonts   +7 more
doaj   +1 more source

Microfluidics for cryopreservation [PDF]

Lab on a Chip, 2009
Minimizing cell damage throughout the cryopreservation process is critical to enhance the overall outcome. Osmotic shock sustained during the loading and unloading of cryoprotectants (CPAs) is a major source of cell damage during the cryopreservation process.
Young S, Song, Sangjun, Moon, Leon, Hulli, Syed K, Hasan, Emre, Kayaalp, Utkan, Demirci   +5 more
openaire   +2 more sources

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source

Culture of pluripotent stem cells in microscale droplets modulates differentiation and tissue patterning towards organoids on chip

Stem Cell Research & Therapy
Background The differentiation of pluripotent stem cells (PSCs) and their self-organization into organoids are influenced by cell-cell interactions mediated by contacts and secreted molecules.
Nadia Vertti-Quintero, Clara Delahousse, Andrey Aristov, Tatiana Traboulsi, Jack-Christophe Cossec, Charles N. Baroud, Sébastien Sart   +6 more
doaj   +1 more source

Development of a New Lab-on-Paper Microfluidics Platform Using Bi-Material Cantilever Actuators for ELISA on Paper

Biosensors, 2023
In this paper, we present a novel and cost-effective lab-on-paper microfluidics platform for performing ELISA autonomously, with no user intervention beyond adding the sample.
Hojat Heidari-Bafroui, Ashutosh Kumar, Cameron Hahn, Nicholas Scholz, Amer Charbaji, Nassim Rahmani, Constantine Anagnostopoulos, Mohammad Faghri   +7 more
doaj   +1 more source