Results 1 to 10 of about 59,493 (294)
The influence of microinjection parameters on cell survival and procedure efficiency
MethodsX, 2023 Microinjection is a method commonly used to deliver various substances into cells. The procedure is performed on a widefield microscope stage using fine glass needle to penetrate the cell membrane. Microinjection can be carried out using a manual or semi-
Joanna Hajduk +3 more
doaj +2 more sources
Development of a Stage- and Species-Specific RNAi System for Molecular Insights in Trichogramma Wasps [PDF]
InsectsTrichogramma wasps, egg parasitoids widely used to control lepidopteran pests, have long eluded in-depth molecular mechanistic studies due to their minute size and genetic tool scarcity. While previous RNAi efforts were restricted to T.
Zelong Yang +11 more
doaj +2 more sources
Frontiers in Marine Science, 2022 The origin and migration of primordial germ cells (PGCs) were regulated by miRNA during embryogenesis, but the underlying mechanism in marine teleost species was still unclear.
Bo Wang +12 more
doaj +1 more source
Frontiers in Genetics, 2021 Genome editing in pigs has been made efficient, practical, and economically viable by the CRISPR/Cas9 platform, representing a promising new era in translational modeling of human disease for research and preclinical development of therapies and devices.
Clifford Dustin Rubinstein +11 more
doaj +1 more source
Using Electroporation to Improve and Accelerate Zebrafish Embryo Toxicity Testing
Micromachines, 2023 Zebrafish have emerged as a useful model for biomedical research and have been used in environmental toxicology studies. However, the presence of the chorion during the embryo stage limits cellular exposure to toxic elements and creates the possibility ...
Nusrat Tazin +3 more
doaj +1 more source
Multiple gene editing in porcine embryos using a combination of microinjection, electroporation, and transfection methods [PDF]
Veterinary World, 2022 Background and Aim: Mosaicism – the presence of both wild-type and mutant alleles – is a serious problem for zygotic gene modification through gene editing using the Clustered regularly interspaced short palindromic repeats-Cas9 (CRISPR/ Cas9) system ...
Quynh Anh Le +8 more
doaj +1 more source
A Review of Recent Advances in Robotic Cell Microinjection
IEEE Access, 2020 Cell microinjection is a direct and effective way to transfer external materials into the cell. In the field of modern biomedicine, cell microinjection is very important, no matter in genetics, reproductive health, tumor therapy or others related ...
Ziqiang Chi, Qingsong Xu, Limin Zhu
doaj +1 more source
Infrared Laser-Based Single Cell Permeabilization by Plasma Membrane Temperature Gradients
Membranes, 2022 Single cell microinjection provides precise tuning of the volume and timing of delivery into the treated cells; however, it also introduces workflow complexity that requires highly skilled operators and specialized equipment.
Allen L. Garner +2 more
doaj +1 more source
Accessing Mitochondrial Protein Import in Living Cells by Protein Microinjection
Frontiers in Cell and Developmental Biology, 2021 Mitochondrial protein biogenesis relies almost exclusively on the expression of nuclear-encoded polypeptides. The current model postulates that most of these proteins have to be delivered to their final mitochondrial destination after their synthesis in ...
Andrey Bogorodskiy +26 more
doaj +1 more source
Frontiers in Cell and Developmental Biology, 2021 Utilizing microinjection to introduce biological molecules such as DNA, mRNA, siRNA, and proteins into the cell is well established to study oocyte maturation and early embryo development in vitro. However, microinjection is an empirical technology.
Lei-Ning Chen +18 more
doaj +1 more source