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Development of a Stage- and Species-Specific RNAi System for Molecular Insights in Trichogramma Wasps [PDF]
InsectsTrichogramma wasps, egg parasitoids widely used to control lepidopteran pests, have long eluded in-depth molecular mechanistic studies due to their minute size and genetic tool scarcity. While previous RNAi efforts were restricted to T.
Zelong Yang +11 more
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Triple gene editing in porcine embryos using electroporation alone or in combination with microinjection [PDF]
Veterinary World, 2022 Background and Aim: We previously developed the gene-editing by electroporation (EP) of Cas9 protein method, in which the CRISPR/Cas9 system was introduced into porcine in vitro fertilized (IVF) zygotes through EP to disrupt a target gene.
Zhao Namula +8 more
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Frontiers in Marine Science, 2022 The origin and migration of primordial germ cells (PGCs) were regulated by miRNA during embryogenesis, but the underlying mechanism in marine teleost species was still unclear.
Bo Wang +12 more
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Frontiers in Genetics, 2021 Genome editing in pigs has been made efficient, practical, and economically viable by the CRISPR/Cas9 platform, representing a promising new era in translational modeling of human disease for research and preclinical development of therapies and devices.
Clifford Dustin Rubinstein +11 more
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Using Electroporation to Improve and Accelerate Zebrafish Embryo Toxicity Testing
Micromachines, 2023 Zebrafish have emerged as a useful model for biomedical research and have been used in environmental toxicology studies. However, the presence of the chorion during the embryo stage limits cellular exposure to toxic elements and creates the possibility ...
Nusrat Tazin +3 more
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A Review of Recent Advances in Robotic Cell Microinjection
IEEE Access, 2020 Cell microinjection is a direct and effective way to transfer external materials into the cell. In the field of modern biomedicine, cell microinjection is very important, no matter in genetics, reproductive health, tumor therapy or others related ...
Ziqiang Chi, Qingsong Xu, Limin Zhu
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The influence of microinjection parameters on cell survival and procedure efficiency
MethodsX, 2023 Microinjection is a method commonly used to deliver various substances into cells. The procedure is performed on a widefield microscope stage using fine glass needle to penetrate the cell membrane. Microinjection can be carried out using a manual or semi-
Joanna Hajduk +3 more
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Microinjection Method for Analyzing Zebrafish Early Stage Oocytes
Frontiers in Cell and Developmental Biology, 2021 Maternal factors which accumulate and establish oocyte polarity during the early stages of oogenesis play key roles in embryonic development, as well as germ cell formation. However, vertebrate oogenesis, especially early stages of oogenesis, is not well
Manami Kobayashi +2 more
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Efficient hyperactive piggyBac transgenesis in Plodia pantry moths
Frontiers in Genome Editing, 2022 While piggyBac transposon-based transgenesis is widely used in various emerging model organisms, its relatively low transposition rate in butterflies and moths has hindered its use for routine genetic transformation in Lepidoptera.
Christa Heryanto +2 more
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CRISPR/Cas9 establishment-mediated targeted mutagenesis in Macrobrachium nipponense
Frontiers in Physiology, 2023 Introduction: CRISPR/Cas9 is a gene-editing technology which could specifically cleave dsDNA and induce target gene mutation. CRISPR/Cas9 has been widely used in gene functional studies in many fields, such as medicine, biology, and agriculture due to ...
Hui Qiao +10 more
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