Results 11 to 20 of about 99,497 (338)
Microinjecting Cells Using a Constant-Flow Microinjection System [PDF]
Cold Spring Harbor Protocols, 2011 INTRODUCTIONThe efficient delivery of exogenous DNA to cells for expression and function studies is an essential technique of modern cell biology, and direct delivery of genetic material by microinjection remains a reliable means of transfection. In the system described here a constant flow of sample is delivered from the tip of the pipette, and the ...
Joshua Z. Gasiorowski, David A. Dean
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Components of a Microinjection System [PDF]
Cold Spring Harbor Protocols, 2011 INTRODUCTIONDirect-pressure microinjection with a micropipette is an essential tool for introducing a variety of impermeant substances into the cytoplasm or nucleus of plant and animal cells. Microinjection remains the most direct method to gain insight into the function and dynamics of intracellular components, to produce transgenic animals, or to ...
Gary G. Borisy +2 more
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Frontiers in Physiology, 2022 Orexins are neuropeptides originating from the hypothalamus that serve broad physiological roles, including the regulation of autonomic function, sleep-wake states, arousal and breathing.
Adrienn G. Varga +6 more
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CRISPR/Cas9 mediated gene editing in non-model nematode Panagrolaimus sp. PS1159
Frontiers in Genome Editing, 2023 The phylum Nematoda harbors a huge diversity of species in a broad range of ecosystems and habitats. Nematodes share a largely conserved Bauplan but major differences have been found in early developmental processes. The development of the nematode model
Viktoria Hellekes +6 more
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GABA\u3csub\u3eA\u3c/sub\u3e and excitatory amino acid receptors in dorsomedial hypothalamus and heart rate in rats [PDF]
, 1991 We have previously shown that microinjection of drugs that interfere with the function of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) into the hypothalamus produces cardiorespiratory and behavioral changes resembling those seen in ...
DiMicco, Joseph A., Soltis, Robert P.
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浙江大学学报. 农业与生命科学版, 2008 Generation of chimera mice with human mitochondria carrying A3243G mutation by microinjection technique could provide an ideal model for studying how xeno-mitochondrial DNA is transmitted to following generations and distributed in various tissues and ...
LI Ji-xia, WANG Ju, LIU Yi-nong
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RNA Interference of Genes Encoding the Vacuolar-ATPase in Liriomyza trifolii
Insects, 2021 The leafminer fly, Liriomyza trifolii, is an invasive pest of vegetable and horticultural crops in China. In this study, a microinjection method based on dsRNA was developed for RNA interference (RNAi) in L. trifolii using genes encoding vacuolar-ATPase (
Ya-Wen Chang +4 more
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Interaction of hypothalamic GABA\u3csub\u3eA\u3c/sub\u3e and excitatory amino acid receptors controlling heart rate in rats [PDF]
, 1991 We have previously shown that microinjection of drugs that impair gamma-aminobutyric acid (GABA)-mediated synaptic inhibition into the dorsomedial hypothalamus (DMH) of rats generates cardiovascular and behavioral changes that mimic the response to ...
DiMicco, Joseph A., Soltis, Robert P.
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Gene Delivery System Using Droplet Injector and Temperature-Controlled Planarian Holder
Inventions, 2018 A microinjection system for gene delivery to a planarian was presented with materials widely used by manufacturers. The system consists of a nanoliter droplet generator/injector and a planarian holder.
Michael Lee +3 more
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Shiyan dongwu yu bijiao yixue, 2023 Objective Construction of a negative control line for the Drosophila transgenic system based on ΦC31 integrase and vector plasmid pUASTattB to provide a more scientific negative control for transgenic Drosophila research experiments.Methods The vector ...
XU Longmei +3 more
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