Results 11 to 20 of about 59,493 (294)
浙江大学学报. 农业与生命科学版, 2008 Generation of chimera mice with human mitochondria carrying A3243G mutation by microinjection technique could provide an ideal model for studying how xeno-mitochondrial DNA is transmitted to following generations and distributed in various tissues and ...
LI Ji-xia, WANG Ju, LIU Yi-nong
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Gene Delivery System Using Droplet Injector and Temperature-Controlled Planarian Holder
Inventions, 2018 A microinjection system for gene delivery to a planarian was presented with materials widely used by manufacturers. The system consists of a nanoliter droplet generator/injector and a planarian holder.
Michael Lee +3 more
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Frontiers in Physiology, 2019 Yeast-like symbiotes (YLS), harbored in the abdomen fat-body cells of the rice brown planthopper (BPH), Nilaparvata lugens Stål (Hemiptera: Delphacidae), are vital to the growth and reproduction of their host. It is feasible to manipulate BPH infestation
Xuping Shentu +3 more
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Triple gene editing in porcine embryos using electroporation alone or in combination with microinjection [PDF]
Veterinary World, 2022 Background and Aim: We previously developed the gene-editing by electroporation (EP) of Cas9 protein method, in which the CRISPR/Cas9 system was introduced into porcine in vitro fertilized (IVF) zygotes through EP to disrupt a target gene.
Zhao Namula +8 more
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Microinjecting Cells Using a Constant-Flow Microinjection System [PDF]
Cold Spring Harbor Protocols, 2011 INTRODUCTIONThe efficient delivery of exogenous DNA to cells for expression and function studies is an essential technique of modern cell biology, and direct delivery of genetic material by microinjection remains a reliable means of transfection. In the system described here a constant flow of sample is delivered from the tip of the pipette, and the ...
David A, Dean, Joshua Z, Gasiorowski
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Advanced Biomedical Research, 2022 Background: Glutamate is an important excitatory neurotransmitter in the pedunculopontine tegmental (PPT) nucleus. The cardiovascular effect of glutamate and its non-N-methyl-D-aspartate (NMDA) receptor in the PPT is unknown; therefore, we evaluated ...
Mohammad Reza Hosseiniravesh +5 more
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Microorganisms, 2020 Viroids are single-stranded noncoding RNA molecules of 250–400 nucleotides that cause plant diseases. One of the two families of viroids is Pospiviroidae, the members of which replicate in the nuclei of host cells.
Hyesu Seo, Ying Wang, Woong June Park
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Specific Protein Antigen Delivery to Human Langerhans Cells in Intact Skin
Frontiers in Immunology, 2021 Immune modulating therapies and vaccines are in high demand, not least to the recent global spread of SARS-CoV2. To achieve efficient activation of the immune system, professional antigen presenting cells have proven to be key coordinators of such ...
Mareike Rentzsch +14 more
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CRISPR/Cas9-Mediated Genome Editing and Mutagenesis of EcChi4 in Exopalaemon carinicauda
G3: Genes, Genomes, Genetics, 2016 The development of the type II clustered regularly interspaced short palindromic repeats (CRISPR) system has resulted in the revolution of genetic engineering, and this technology has been applied in the genome editing of various species.
Tianshu Gui +6 more
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Veterinary Medicine and Science, 2021 Despite all the advances in the production of transgenic mice, the production efficiency of these animal models is still low. Given that the expression of developmental genes has a critical role in growth and development of embryo, we determined the ...
Maryam Salimi +7 more
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