Results 261 to 270 of about 59,493 (294)
Some of the next articles are maybe not open access.
Challenges in capillary pressure microinjection
The 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 2005Capillary pressure microinjection is a popular method for the delivery of samples into cells. Needs for automatic reliable microinjection systems are growing, but the current systems do not possess ideal characteristics and many researchers do not even recognize the limits of the current systems.
J, Kuncova, P, Kallio
openaire +2 more sources
Single-Cell Microinjection Technologies
2012Single-cell microinjection, as a mechanical delivery tool, has been used for transferring substances into transfection or infection challenging cells. Here, we discuss the advantages and applications of microinjection, list the materials needed for performing microinjection experiments, and describe the methods of single-cell microinjection into ...
openaire +2 more sources
Microinjection into crane-fly spermatocytes
Biochemistry and Cell Biology, 1993We were successful in microinjecting fluorescently labelled material into crane-fly spermatocytes. In our experiments, we obtained four results. (i) In most attempts, the membrane stretched around the micropipette and prevented entry of fluorescent material, even when the micropipette appeared to be pushed completely through the cell.
B B, Czaban, A, Forer, D A, Wise
openaire +2 more sources
Science, 1966
A technique has been developed for injecting known amounts of liquids into fertilized one-celled mouse eggs by use of a calibrated ocular micrometer superimposed on the terminal regions of the injection pipette. From 128 pronuclear eggs, each injected with 180 or 770 cubic microns of bovine gamma globulin at a concentration of 25 milligrams per ...
openaire +2 more sources
A technique has been developed for injecting known amounts of liquids into fertilized one-celled mouse eggs by use of a calibrated ocular micrometer superimposed on the terminal regions of the injection pipette. From 128 pronuclear eggs, each injected with 180 or 770 cubic microns of bovine gamma globulin at a concentration of 25 milligrams per ...
openaire +2 more sources
Plating Cells for Microinjection
Cold Spring Harbor Protocols, 2006INTRODUCTIONThis protocol describes a method for plating cells for microinjection onto etched coverslips. The coverslips for microinjection must be marked so that microinjected cells can be identified at time points after injection. A procedure for etching coverslips is given below; alternatively, pre-etched coverslips can be purchased at slightly ...
openaire +2 more sources
Implantation of Microinjected Eggs
2003Microinjected eggs are normally incubated overnight in Ml6 microdrop culture at 37°C in CO₂ until 1400 h. By this time the eggs will have developed as far as the two-cell stage. The eggs are then implanted into surrogate mothers. As the membrane covering the oviduct of the rat is much tougher than the mouse, we normally cut it with a very fine pair of ...
openaire +2 more sources