Results 21 to 30 of about 59,493 (294)
Molecules, 2022 Zebrafish (ZF; Danio rerio) larvae have become a popular in vivo model in drug metabolism studies. Here, we investigated the metabolism of methyl 2-[1-(4-fluorobutyl)-1H-indazole-3-carboxamido]-3,3-dimethylbutanoate (4F-MDMB-BINACA) in ZF larvae after ...
Yu Mi Park +5 more
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Microfluidic Device for Microinjection of Caenorhabditis elegans
Micromachines, 2020 Microinjection is an established and reliable method to deliver transgenic constructs and other reagents to specific locations in C. elegans worms. Specifically, microinjection of a desired DNA construct into the distal gonad is the most widely used ...
Reza Ghaemi +3 more
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A Review of Automated Microinjection of Zebrafish Embryos
Micromachines, 2018 Cell microinjection is a technique of precise delivery of substances into cells and is widely used for studying cell transfection, signaling pathways, and organelle functions.
Yuliang Zhao +5 more
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The Role of Nod Factor Substituents in Actin Cytoskeleton Rearrangements in Phaseolus vulgaris
Molecular Plant-Microbe Interactions, 2003 In order to define the symbiotic role of some of the chemical substituents in the Rhizobium etli Nod factors (NFs), we purified Nod metabolites secreted by the SM25 strain, which carries most of the nodulation genes, and SM17 with an insertion in nodS ...
Luis Cárdenas +5 more
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Microinjection Method for Analyzing Zebrafish Early Stage Oocytes
Frontiers in Cell and Developmental Biology, 2021 Maternal factors which accumulate and establish oocyte polarity during the early stages of oogenesis play key roles in embryonic development, as well as germ cell formation. However, vertebrate oogenesis, especially early stages of oogenesis, is not well
Manami Kobayashi +2 more
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Efficient hyperactive piggyBac transgenesis in Plodia pantry moths
Frontiers in Genome Editing, 2022 While piggyBac transposon-based transgenesis is widely used in various emerging model organisms, its relatively low transposition rate in butterflies and moths has hindered its use for routine genetic transformation in Lepidoptera.
Christa Heryanto +2 more
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CRISPR/Cas9 establishment-mediated targeted mutagenesis in Macrobrachium nipponense
Frontiers in Physiology, 2023 Introduction: CRISPR/Cas9 is a gene-editing technology which could specifically cleave dsDNA and induce target gene mutation. CRISPR/Cas9 has been widely used in gene functional studies in many fields, such as medicine, biology, and agriculture due to ...
Hui Qiao +10 more
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Genetics and Molecular Biology, 2007 In fish, microinjection is the method most frequently used for gene transfer. However, due to delayed transgene integration this technique almost invariably produces mosaic individuals and if the gene is not integrated into germ cells its transmission to
Márcio de Azevedo Figueiredo +3 more
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Optimization of Existing RNA Visualization Methods Reveals Novel Dendritic mRNA Dynamics
Frontiers in Bioscience-LandmarkBackground: Spatial-temporal control of mRNA translation in dendrites is important for synaptic plasticity. In response to pre-synaptic stimuli, local mRNA translation can be rapidly triggered near stimulated synapses to supply the ...
Ivan J. Cohen +4 more
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The Making of Transgenic Drosophila guttifera
Methods and Protocols, 2020 The complex color patterns on the wings and body of Drosophila guttifera (D. guttifera) are emerging as model systems for studying evolutionary and developmental processes.
Mujeeb Shittu +3 more
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