Results 301 to 310 of about 99,497 (338)
Some of the next articles are maybe not open access.
Cloning and Stem Cells, 2001
Last year marked the 20th anniversary of the invention of the term "transgenic" and the development of pronuclear microinjection, a straightforward technique designed to transfer genetic information from nearly any living organism to mammals. After two decades of use, pronuclear microinjection protocols have changed little from the reliable, if not ...
openaire +2 more sources
Last year marked the 20th anniversary of the invention of the term "transgenic" and the development of pronuclear microinjection, a straightforward technique designed to transfer genetic information from nearly any living organism to mammals. After two decades of use, pronuclear microinjection protocols have changed little from the reliable, if not ...
openaire +2 more sources
Microinjecting Cells Using a Pulsed-Flow Microinjection System
Cold Spring Harbor Protocols, 2011INTRODUCTIONThe efficient delivery of exogenous DNA to cells for expression and function studies is an essential technique of modern cell biology, and direct delivery of genetic material by microinjection remains a reliable means of transfection. A pulsed-flow microinjection system, such as the Eppendorf InjectMan and FemtoJet systems, permits a high ...
David A. Dean, Joshua Z. Gasiorowski
openaire +3 more sources
Generation of Chimeras by Microinjection
2009Since the technique of introducing a targeted mutation ('gene targeting') into the mouse genome was published almost 20 years ago (Cell 51:503-512, 1987), the number of mouse mutants (mouse models) is increasing, especially after the advent of the full mouse genomic sequence in 2002 and the human genomic sequences in 2003 that reveals more and more ...
Christian Klasen, Anne Plück
openaire +3 more sources
Microinjection Methods for Neutrophils
2014The ability to microinject substances into the cytosol of living neutrophils opens the possibility of manipulating the chemistry within the cell and also of monitoring changes using indicators which otherwise cannot be introduced into the cell. However, neutrophils cannot be microinjected by the conventional glass pipette insertion method.
Iraj Laffafian +3 more
openaire +3 more sources
[28] Microinjection of antibodies
1995Publisher Summary This chapter describes the use of the microinjection technique as a way to deliver immunopurified antibodies into the cytoplasm of cells in culture. The success in the microinjection of antibodies for neutralization purposes relies mainly on the quality of the antibodies used.
openaire +3 more sources
Microinjection of Protein Samples
Cold Spring Harbor Protocols, 2007INTRODUCTIONThis protocol describes a method for microinjecting proteins into the nucleus or cytoplasm of adherent cells. Microinjection equipment can be obtained from a number of suppliers; this protocol has been used with the Narishige IM-200 air pressure regulator and the Leitz micromanipulator.
Yulia Komarova +2 more
openaire +3 more sources
Mosquito Embryo Microinjection
Cold Spring Harbor Protocols, 2023Genetically modified (GM) mosquitoes are an important tool in the fight against mosquito-borne disease, both indirectly through their use in research investigating host–pathogen interaction, mosquito olfaction, and anthropomorphic behavior and in future direct uses for suppression and possibly eradication through sterile insect technique (SIT) and/or ...
openaire +2 more sources
Microinjection of Mouse Eggs [PDF]
Science, 1966 A technique has been developed for injecting known amounts of liquids into fertilized one-celled mouse eggs by use of a calibrated ocular micrometer superimposed on the terminal regions of the injection pipette. From 128 pronuclear eggs, each injected with 180 or 770 cubic microns of bovine gamma globulin at a concentration of 25 milligrams per ...
openaire +2 more sources
Microinjection of Xenopus Oocytes
Cold Spring Harbor Protocols, 2010INTRODUCTIONXenopus oocytes can be injected with messenger RNA (mRNA) or DNA constructs into the cytoplasm or nucleus, respectively. The cytoplasm can withstand the introduction of up to 50 nL of injected material, and the nucleus can tolerate up to 20 nL.
Richard M. Harland +2 more
openaire +3 more sources