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Chapter 12 Microinjection of Mitotic Cells
1998Publisher Summary This chapter reviews the method of direct needle microinjection and discusses the application of this method in the study of mitotic cells. As virtually any molecule of interest that can be purified and obtained at an appropriate concentration can be injected into the living mitotic cell, this method can be utilized to study how ...
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Microinjection of Living Adherent Cells by Using a Semi-automatic Microinjection System
Alternatives to Laboratory Animals, 2004Testing in vitro is an alternative to animal experimentation. The capillary pressure microinjection technique is a supporting technology for efficient in vitro testing. The main benefit of the technique is the possibility of injecting large molecules into a single living cell.
Pasi Kallio, Katrin Viigipuu
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Challenges in capillary pressure microinjection
The 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 2005Capillary pressure microinjection is a popular method for the delivery of samples into cells. Needs for automatic reliable microinjection systems are growing, but the current systems do not possess ideal characteristics and many researchers do not even recognize the limits of the current systems.
Pasi Kallio, J. Kuncova
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Plating Cells for Microinjection
Cold Spring Harbor Protocols, 2006INTRODUCTIONThis protocol describes a method for plating cells for microinjection onto etched coverslips. The coverslips for microinjection must be marked so that microinjected cells can be identified at time points after injection. A procedure for etching coverslips is given below; alternatively, pre-etched coverslips can be purchased at slightly ...
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Microinjection of calcium into droplets of aequorin
Proceedings of the Royal Society of London. Series B. Biological Sciences, 1975Calcium ions were microinjected, ionophoretically or by pressure, into droplets of the photoprotein aequorin. For pulses less than about 1 s in duration, the amplitude and rate of rise of the light emitted as a result of the combination of calcium with aequorin, were directly proportional to the ionophoretic current flowing through the Ca pipette ...
Ricardo Miledi, K. Kusano, J. Stinnakre
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Microinjection into crane-fly spermatocytes
Biochemistry and Cell Biology, 1993We were successful in microinjecting fluorescently labelled material into crane-fly spermatocytes. In our experiments, we obtained four results. (i) In most attempts, the membrane stretched around the micropipette and prevented entry of fluorescent material, even when the micropipette appeared to be pushed completely through the cell.
Arthur Forer +2 more
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Rapid Microinjection of Fertilized Eggs
2004The chapter focuses on methods for using a picospritzer to inject large numbers of eggs that have been immobilized in open plastic dishes. The microinjection of reagents into eggs and early embryos aids in analyzing many cellular and developmental processes and is essential in studying cell lineages and cell fate specification, gene regulation, gene ...
Charles A. Ettensohn, Melani S. Cheers
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Development of a Vibratory Microinjection Method
The International Journal of Artificial Organs, 2003To reduce cellular damage by pronuclear microinjection and nuclear transfer, we have recently developed a vibratory microinjection method. A micropipette was fixed to a piezoelectric ceramic with a resonance frequency of 70 kHz. When this micropipette was vibrated, it easily entered a mouse-fertilized egg without any sharp depression of the cell body,
Y Arai +5 more
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Microinjection - a tool to study gravitropism
Advances in Space Research, 2003Despite extensive studies on plant gravitropism this phenomenon is still poorly understood. The separation of gravity sensing, signal transduction and response is a common concept but especially the mechanism of gravisensing remains unclear. This paper focuses on microinjection as powerful tool to investigate gravisensing in plants.
Karl H. Hasenstein, P. Scherp
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Implantation of Microinjected Eggs
2003Microinjected eggs are normally incubated overnight in Ml6 microdrop culture at 37°C in CO₂ until 1400 h. By this time the eggs will have developed as far as the two-cell stage. The eggs are then implanted into surrogate mothers. As the membrane covering the oviduct of the rat is much tougher than the mouse, we normally cut it with a very fine pair of ...
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