Results 31 to 40 of about 59,493 (294)

Activation of orexin-2 receptors in the Kӧlliker-Fuse nucleus of anesthetized mice leads to transient slowing of respiratory rate

Frontiers in Physiology, 2022
Orexins are neuropeptides originating from the hypothalamus that serve broad physiological roles, including the regulation of autonomic function, sleep-wake states, arousal and breathing.
Adrienn G. Varga, Adrienn G. Varga, Jessica R. Whitaker-Fornek, Jessica R. Whitaker-Fornek, Sebastian N. Maletz, Erica S. Levitt, Erica S. Levitt   +6 more
doaj   +1 more source

CRISPR/Cas9 mediated gene editing in non-model nematode Panagrolaimus sp. PS1159

Frontiers in Genome Editing, 2023
The phylum Nematoda harbors a huge diversity of species in a broad range of ecosystems and habitats. Nematodes share a largely conserved Bauplan but major differences have been found in early developmental processes. The development of the nematode model
Viktoria Hellekes, Viktoria Hellekes, Denise Claus, Johanna Seiler, Felix Illner, Philipp H. Schiffer, Michael Kroiher   +6 more
doaj   +1 more source

TMC4 localizes to multiple taste cell types in the mouse taste papillae

FEBS Open Bio, EarlyView.
Transmembrane channel‐like 4 (TMC4), a voltage‐dependent chloride channel, plays a critical role in amiloride‐insensitive salty taste transduction. TMC4 is broadly expressed in all mature taste cell types, suggesting a possible involvement of multiple cell types in this pathway.
Momo Murata, Yoshikazu Saito, Yoichi Kasahara, Wataru Fujii, Takumi Misaka, Tomiko Asakura, Masataka Narukawa   +6 more
wiley   +1 more source

RNA Interference of Genes Encoding the Vacuolar-ATPase in Liriomyza trifolii

Insects, 2021
The leafminer fly, Liriomyza trifolii, is an invasive pest of vegetable and horticultural crops in China. In this study, a microinjection method based on dsRNA was developed for RNA interference (RNAi) in L. trifolii using genes encoding vacuolar-ATPase (
Ya-Wen Chang, Yu-Cheng Wang, Xiao-Xiang Zhang, Junaid Iqbal, Yu-Zhou Du   +4 more
doaj   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Generation of 12 Drosophila Transgenic Negative Control Lines Based on Site-specific ΦC31 Integrase and pUASTattB Vector

Shiyan dongwu yu bijiao yixue, 2023
Objective Construction of a negative control line for the Drosophila transgenic system based on ΦC31 integrase and vector plasmid pUASTattB to provide a more scientific negative control for transgenic Drosophila research experiments.Methods The vector ...
XU Longmei, SHEN Ruling, FAN Chun, WU Wei   +3 more
doaj   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

Drug Administration Routes Impact the Metabolism of a Synthetic Cannabinoid in the Zebrafish Larvae Model

Molecules, 2020
Zebrafish (Danio rerio) larvae have gained attention as a valid model to study in vivo drug metabolism and to predict human metabolism. The microinjection of compounds, oligonucleotides, or pathogens into zebrafish embryos at an early developmental stage
Yu Mi Park, Markus R. Meyer, Rolf Müller, Jennifer Herrmann   +3 more
doaj   +1 more source

Self‐Powered Permeable Electronic Dressing for Exudate Management, Electrostimulation and Drug Delivery in Chronic Wound Healing

Advanced Functional Materials, EarlyView.
This study develops a self‐powered permeable electronic dressing (SPED) that synergistically integrates exudate management, electrical stimulation, and on‐demand drug delivery. The dressing effectively absorbs exudate while demonstrating potent antibacterial activity and accelerated tissue regeneration in diabetic mouse models, thereby promoting ...
Jiaheng Liang, Jingjing Fu, Jin Chai, Hongyu Jiang, Fan Chen, Yufei Zhang, Weihao Zhao, Xian Song, Zhihao Zhou, Lingtao Fang, Qiyao Huang, Peng Li, Zijian Zheng   +12 more
wiley   +1 more source

Expression of Genes Involved in Anthracnose Resistance in Chili (Capsicum baccatum) ‘PBC80’-Derived Recombinant Inbred Lines

Pathogens, 2023
Chili anthracnose has long been a threat to chili production worldwide. Capsicum baccatum ‘PBC80’ has been identified as a source of resistance to anthracnose.
Wassana Kethom, Paul W. J. Taylor, Orarat Mongkolporn   +2 more
doaj   +1 more source