CRISPR/Cas9-Mediated Genome Editing and Mutagenesis of EcChi4 in Exopalaemon carinicauda
G3: Genes, Genomes, Genetics, 2016 The development of the type II clustered regularly interspaced short palindromic repeats (CRISPR) system has resulted in the revolution of genetic engineering, and this technology has been applied in the genome editing of various species.
Tianshu Gui +6 more
doaj +1 more source
Comparative susceptibility of mosquito populations in North Queensland, Australia to oral infection with dengue virus. [PDF]
, 2014 Dengue is the most prevalent arthropod-borne virus, with at least 40% of the world's population at risk of infection each year. In Australia, dengue is not endemic, but viremic travelers trigger outbreaks involving hundreds of cases.
Anderson +47 more
core +2 more sources
Interaction of GABA and Excitatory Amino Acids in the Basolateral Amygdala: Role in Cardiovascular Regulation [PDF]
, 1997 Activation of the amygdala in rats produces cardiovascular changes that include increases in heart rate and arterial pressure as well as behavioral changes characteristic of emotional arousal.
Cook, Jennifer C. +3 more
core +2 more sources
A Review of Automated Microinjection of Zebrafish Embryos
Micromachines, 2018 Cell microinjection is a technique of precise delivery of substances into cells and is widely used for studying cell transfection, signaling pathways, and organelle functions.
Yuliang Zhao +5 more
doaj +1 more source
Infrared Laser-Based Single Cell Permeabilization by Plasma Membrane Temperature Gradients
Membranes, 2022 Single cell microinjection provides precise tuning of the volume and timing of delivery into the treated cells; however, it also introduces workflow complexity that requires highly skilled operators and specialized equipment.
Allen L. Garner +2 more
doaj +1 more source
Primary cultures of chick osteocytes retain functional gap junctions between osteocytes and between osteocytes and osteoblasts [PDF]
, 2008 The inaccessibility of osteocytes due to their embedment in the calcified bone matrix in vivo has precluded direct demonstration that osteocytes use gap junctions as a means of intercellular communication.
Ishihara, Yoshihito +6 more
core +1 more source
Nuclear inclusion of nontargeted and chromatin-targeted polystyrene beads and plasmid DNA containing nanoparticles [PDF]
, 2011 The nuclear membrane is one of the major cellular barriers in the delivery of plasmid DNA (pDNA). Cell division has a positive influence on the expression efficiency since, at the end of mitosis, pDNA or pDNA containing complexes near the chromatin are ...
De Smedt, Stefaan +5 more
core +1 more source
Advanced Biomedical Research, 2017 Background: Orexins are excitatory neuropeptides which stimulate the central regulatory pathways. Orexins increase the penicillin-induced epileptic activity in rats.
Parichehr Hayatdavoudi +3 more
doaj +1 more source
Accessing Mitochondrial Protein Import in Living Cells by Protein Microinjection
Frontiers in Cell and Developmental Biology, 2021 Mitochondrial protein biogenesis relies almost exclusively on the expression of nuclear-encoded polypeptides. The current model postulates that most of these proteins have to be delivered to their final mitochondrial destination after their synthesis in ...
Andrey Bogorodskiy +26 more
doaj +1 more source
Cortical granule exocytosis is mediated by alpha-SNAP and N-Ethilmaleimide sensitive factor in mouse eggs [PDF]
, 2015 Cortical granule exocytosis (CGE), also known as cortical reaction, is a calciumregulated secretion that represents a membrane fusion process during meiotic cell division of eggs.
Bello, Oscar Daniel +2 more
core +3 more sources