Results 41 to 50 of about 99,497 (338)

High-Survival Rate After Microinjection of Mouse Oocytes and Early Embryos With mRNA by Combining a Tip Pipette and Piezoelectric-Assisted Micromanipulator

Frontiers in Cell and Developmental Biology, 2021
Utilizing microinjection to introduce biological molecules such as DNA, mRNA, siRNA, and proteins into the cell is well established to study oocyte maturation and early embryo development in vitro. However, microinjection is an empirical technology.
Lei-Ning Chen, Lei-Ning Chen, Xiao-Yan Fan, Xiao-Yan Fan, Yi-Tong Liu, Shao-Qing Chen, Feng-Yun Xie, Feng-Yun Xie, Li Zeng, Juan Wen, Jin Li, Jun-Yu Ma, Jun-Yu Ma, Xiang-Hong Ou, Xiang-Hong Ou, Shi-Ming Luo, Shi-Ming Luo, Lei Guo, Lei Guo   +18 more
doaj   +1 more source

The Making of Transgenic Drosophila guttifera

Methods and Protocols, 2020
The complex color patterns on the wings and body of Drosophila guttifera (D. guttifera) are emerging as model systems for studying evolutionary and developmental processes.
Mujeeb Shittu, Tessa Steenwinkel, Shigeyuki Koshikawa, Thomas Werner   +3 more
doaj   +1 more source

Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP [PDF]

, 1999
Raf-1 phosphorylates and activates MEK-1, a kinase that activates the extracellular signal regulated kinases (ERK). This kinase cascade controls the proliferation and differentiation of different cell types.
A Minden, A Sewing, AD Catling, B Hallberg, CJ Marshall, D Woods, DK Grandy, DK Morrison, DW Rose, F Schoentgen, Ferrell, J. E. Jr, Ferrell, J. E. Jr, H Mischak, HJ Schaeffer, JT Bruder, KL Guan, M Kortenjann, OV Gjorup, RM Lavbinsky, RM Tombes, S Häfner, S Li, SA Moodie, UR Rapp, W Kolch, W Kolch   +25 more
core   +1 more source

Microinjection of Xenopus Laevis Oocytes [PDF]

Journal of Visualized Experiments, 2009
Microinjection of Xenopus laevis oocytes followed by thin-sectioning electron microscopy (EM) is an excellent system for studying nucleocytoplasmic transport. Because of its large nucleus and high density of nuclear pore complexes (NPCs), nuclear transport can be easily visualized in the Xenopus oocyte.
Sarah Cohen, Shelly Au, Nelly Panté
openaire   +2 more sources

Irisin evokes bradycardia by activating cardiac-projecting neurons of nucleus ambiguus. [PDF]

, 2015
Irisin is a newly identified hormone induced in muscle and adipose tissues by physical activity. This protein and its encoding gene have been identified in the brain; in addition, the precursor for irisin, FNDC5, can cross the blood-brain barrier.
Brailoiu, Eugen, Brailoiu, Gabriela Cristina, Deliu, Elena, Sporici, Romeo A   +3 more
core   +2 more sources

Copper-containing mesoporous bioactive glass promotes angiogenesis in an in vivo zebrafish model [PDF]

, 2017
The osteogenic and angiogenic responses of organisms to the ionic products of degradation of bioactive glasses (BGs) are being intensively investigated. The promotion of angiogenesis by copper (Cu) has been known for more than three decades. This element
Aguiar, Arkudas, Aránzazu Díaz-Cuenca, Chang, Childs, Craig, Cui, Day, Dooley, Garcia-Olmo, García-Vilas, Geissberger, Gerhardt, Goi, Goldberg, González, Gorustovich, Gospodarowicz, Griffitt, Hagemann, Handel, Hench, Hench, Hoppe, Hu, Kari, Kong, Lawson, Li, Li, Lilian B. Romero-Sánchez, Manuel Marí-Beffa, Martin, Mckeown, Melet, Miguel Ángel Medina, Montazerolghaem, Naderi, Neumann, Oliveira, Paloma Carrillo, Parng, Perez, Pérez-Robles, Rahaman, Raju, Ramiro-Gutiérrez, Ramiro-Gutiérrez, Rath, Romero-Sánchez, Schuermann, Sen, Shing, Soncin, Staton, Stähli, Subbiah, Thomas, Thompson, Tienderson, Urso, Vallet-Regí, Vallet-Regí, Wang, Wu, Wu, Wu, Xia, Xiao, Xynos, Yan, Zhai   +71 more
core   +2 more sources

Optimization of Existing RNA Visualization Methods Reveals Novel Dendritic mRNA Dynamics

Frontiers in Bioscience-Landmark
Background: Spatial-temporal control of mRNA translation in dendrites is important for synaptic plasticity. In response to pre-synaptic stimuli, local mRNA translation can be rapidly triggered near stimulated synapses to supply the ...
Ivan J. Cohen, Tianhui Zhu, Marcus Ng, Hao Wu, Jason Dictenberg   +4 more
doaj   +1 more source

Involvement of ras p2I in Neurotrophin-induced Response of Sensory, but Not Sympathetic Neurons [PDF]

, 1993
Little is known about the signal transduction mechanisms involved in the response to neurotrophins and other neurotrophic factors in neurons, beyond the activation of the tyrosine kinase activity of the neurotrophin receptors belonging to the trk family.
Barde, Y. A., Borasio, Gian Domenico, Heumann, R., Markus, A., Wittinghofer, A.   +4 more
core   +3 more sources

Microinjection of mRNAs and Oligonucleotides [PDF]

Cold Spring Harbor Protocols, 2018
Microinjecting lineage tracers into a single blastomere in the normal, intact embryo identifies the repertoire of cell types derived from it. In order to reveal the full developmental potential of that blastomere or identify the mechanisms by which its fate is determined, one needs to modify its gene expression under controlled experimental conditions.
openaire   +3 more sources

Versatile Cell Penetrating Peptide for Multimodal CRISPR Gene Editing in Primary Stem Cells

Advanced Functional Materials, EarlyView.
CRISPR machinery in diverse molecular formats (DNA, RNA, and ribonucleic protein) is complexed into nanoparticles with the cell‐friendly arginine‐alanine‐leucine‐alanine (RALA) cell‐penetrating peptide. Nanoparticles are delivered to primary mesenchymal stem cells ex vivo or locally in vivo to facilitate multimodal CRISPR gene editing. This RALA‐CRISPR
Joshua P. Graham, Jose Gabriel Castro, Lisette C. Werba, Joseph Amitrano, Luke Fardone, Kevin P. Francis, Anand Ramamurthi, Michael J. Layden, Helen O. McCarthy, Tomas Gonzalez‐Fernandez   +9 more
wiley   +1 more source