Results 61 to 70 of about 99,497 (338)

In Vivo Experimental Study of Noninvasive Insulin Microinjection through Hollow Si Microneedle Array

Micromachines, 2018
An experimental study of in vivo insulin delivery through microinjection by using hollow silicon microneedle array is presented. A case study was carried out on a healthy human subject in vivo to determine the influence of delivery parameters on drug ...
Drago Resnik, Matej Možek, Borut Pečar, Andrej Janež, Vilma Urbančič, Ciprian Iliescu, Danilo Vrtačnik   +6 more
doaj   +1 more source

The effect of CRISPR constructs microinjection on the expression of developmental genes in Rag1 knocked‐out mice embryo

Veterinary Medicine and Science, 2021
Despite all the advances in the production of transgenic mice, the production efficiency of these animal models is still low. Given that the expression of developmental genes has a critical role in growth and development of embryo, we determined the ...
Maryam Salimi, Abolfazl Shirazi, Koushan Sineh Sepehr, Mohsen Norouzian, Vahid Ebrahimi, Maryam Mehravar, Mohammad Majidi, Mohammad M. Mehrazar   +7 more
doaj   +1 more source

Nanobody‐Decorated Lipid Nanoparticles for Enhanced mRNA Delivery to Tumors In Vivo

Advanced Healthcare Materials, EarlyView.
This study presents a targeted mRNA‐LNP platform engineered with anti‐PSMA nanobodies for prostate cancer therapy. Using different in vivo models, the authors demonstrate selective uptake and mRNA delivery in PSMA+ cancer cells. However, while targeting is enhanced, mRNA delivery efficacy is limited by tissue penetration, tumor necrosis, and immune ...
Pol Escudé Martinez de Castilla, Vincenzo Verdi, Willemijn de Voogt, Mariona Estapé Sentí, Arnold C. Koekman, Julian Rietveld, Sven van Kempen, Qiangbing Yang, Juliette van Merris, Guido Jenster, Martin E. van Royen, Marcel H. Fens, Sander A. A. Kooijmans, Wytske M. van Weerden, Guillaume van Niel, Pieter Vader, Raymond M. Schiffelers   +16 more
wiley   +1 more source

Practical Approaches for Knock-Out Gene Editing in Pigs

Frontiers in Genetics, 2021
Pigs are an important resource for meat production and serve as a model for human diseases. Due to their physiological and anatomical similarities to humans, these animals can recapitulate symptoms of human diseases, becoming an effective model for ...
Laura Daniela Ratner, Laura Daniela Ratner, Gaston Emilio La Motta, Gaston Emilio La Motta, Olinda Briski, Olinda Briski, Daniel Felipe Salamone, Daniel Felipe Salamone, Rafael Fernandez-Martin, Rafael Fernandez-Martin   +9 more
doaj   +1 more source

Development of Protacs to Target Cancer-promoting Proteins for Ubiquitination and Degradation [PDF]

, 2003
The proteome contains hundreds of proteins that in theory could be excellent therapeutic targets for the treatment of human diseases. However, many of these proteins are from functional classes that have never been validated as viable candidates for the ...
Crews, Craig M., Deshaies, Raymond J., Kim, Kyung B., Ransick, Andy, Sakamoto, Kathleen M., Stein, Bernd, Verma, Rati   +6 more
core   +1 more source

Multifunctional Neural Probes Enable Bidirectional Electrical, Optical, and Chemical Recording and Stimulation In Vivo

Advanced Materials, EarlyView.
Convergence drawing is used to create flexible, microscale, multifunctional fiber‐based neural probes. Optimized materials selection enables individual devices to perform neural recording, electrical stimulation, optogenetics, fiber photometry, fluid delivery, and voltammetric neurotransmitter detection in rodents.
Nicolette Driscoll, Marc‐Joseph Antonini, Taylor M. Cannon, Pema Maretich, Greatness Olaitan, Valerie Doan Phi Van, Keisuke Nagao, Atharva Sahasrabudhe, Emmanuel Vargas Paniagua, Ethan J. Frey, Ye Ji Kim, Sydney Hunt, Melissa Hummel, Sanju Mupparaju, Alan Jasanoff, B. Jill Venton, Polina Anikeeva   +16 more
wiley   +1 more source

Assistive System for Microinjection by Presenting Cell Deformation as Surface Tactile Sensation

IEEE Access
Microinjection technology has been widely applied in various fields, including biology, medicine, and intracytoplasmic sperm injection (ICSI). In a typical ICSI environment, all tasks, such as determining the degree of extension, adjusting the depth of ...
Sumiwa Saito, Tadayoshi Aoyama, Yuki Funabora, Masaru Takeuchi, Yasuhisa Hasegawa   +4 more
doaj   +1 more source

Immaturin-Nuclease as a Model System for a Gene-Programmed Sexual Development and Rejuvenescence in Paramecium Life History

Microorganisms, 2022
Fertilization-initiated development and adult-onset aging are standard features in the life history of eukaryotes. In Paramecium, the number of cell divisions after the birth of a new generation is an essential parameter of sexual phase transition and ...
Nobuyuki Haga, Toshinori Usui, Yasuhiro Takenaka, Yuta Chiba, Tomoaki Abe   +4 more
doaj   +1 more source

Divergent modulation of nociception by glutamatergic and GABAergic neuronal subpopulations in the periaqueductal gray [PDF]

, 2017
The ventrolateral periaqueductal gray (vlPAG) constitutes a major descending pain modulatory system and is a crucial site for opioid-induced analgesia. A number of previous studies have demonstrated that glutamate and GABA play critical opposing roles in
Bruchas, Michael R, Copits, Bryan A, Gereau, Robert W, IV, Gomez, Adrian M, Grajales-Reyes, Jose G, O\u27Brien, Daniel E, Samineni, Vijay K, Trigg, Sarah L   +7 more
core   +2 more sources

Droplet‐Based Synthesis of Nanogels for Controlled Drug Delivery via Two Photon Polymerization‐3D Printed Microfluidic Device

Advanced Materials Technologies, EarlyView.
A miniaturized microfluidic device is 3D printed via two‐photon polymerization and used for the droplet‐based microfluidic synthesis of nanogels for controlled drug delivery purposes. Tuning the fluid flow parameters, the microfluidic system enables the modulation of nanogel size and properties, preserving an extremely low polydispersity, beyond the ...
Vladimir Matining, Camillo Colli, Emanuela Jacchetti, Giulia Nicoletti, Laura Rosanò, Manuela Teresa Raimondi, Bianca Maria Colosimo, Emanuele Mauri, Davide Moscatelli   +8 more
wiley   +1 more source