Results 81 to 90 of about 699,968 (272)

Optical Aberrations and Objective Choice in Multicolor Confocal Microscopy

BioTechniques, 2000
Refinements in design have simplified confocal microscopy to the extent that it has become a standard research tool in cell biology. However, as confocal microscopes have become more powerful, they have also become more demanding of their optical ...
Kenneth W. Dunn, Exing Wang
doaj   +1 more source

Towards many colors in FISH on 3D-preserved interphase nuclei [PDF]

, 2006
The article reviews the existing methods of multicolor FISH on nuclear targets, first of all, interphase chromosomes. FISH proper and image acquisition are considered as two related components of a single process.
Albiez, H., Benecletti, P. A., Bolzer, A., Cremer, M., Cremer, T., Joffe, B., Müller, Stefan, Solovei, Irina, Speicher, Michael R., Walter, J.   +9 more
core   +1 more source

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

Clinical applications of corneal confocal microscopy

Clinical Ophthalmology, 2008
Mitra Tavakoli1, Parwez Hossain2, Rayaz A Malik11Division of Cardiovascular Medicine, University of Manchester and Manchester Royal Infirmary, Manchester, UK; 2University of Southampton, Southampton Eye Unit, Southampton General Hospital, Southampton ...
Mitra Tavakoli, Parwez Hossain, Rayaz A Malik   +2 more
doaj  

Electron microscopic measurement of the size of the optical focus in laser scanning microscopy [PDF]

, 2012
We describe a method for measuring the lateral focal spot size of a multiphoton laser scanning microscope (LSM) with unprecedented accuracy. A specimen consisting of an aluminum film deposited on a glass coverslip was brought into focus in a LSM and the ...
Alison McDonald, Ditchburn, Gail McConnell, Inoué, Siegman, William B. Amos   +5 more
core   +1 more source

Alcohol‐induced altered glycans in human tracheal epithelial cells promote bacterial adhesion

FEBS Open Bio, EarlyView.
Alcohol induces altered glycans to promote bacteria adhesion. Heavy alcohol drinking is known to increase the risk of bacterial pneumonia. However, the link between alcohol levels and risk of infection remains underexplored. Recently, we found that alcohol induced α2‐6sialo mucin O‐glycans in human tracheobronchial epithelial cells, which mediated the ...
Pi‐Wan Cheng, Souvik Datta, Derrick R. Samuelson   +2 more
wiley   +1 more source

Microscopy study of ripples created on steel surface by use of ultra short laser pulses [PDF]

, 2010
This paper concentrates on ripples on the surface of steel that arise from lasermaterial interaction. In particular we have observed two different sets of ripples on steel samples that were machined by 210 fs laser pulses with 800 nm wavelength at normal
Hosson, J.Th.M. de, Huis in 't Veld, A.J., Mitko, V.S., Ocelik, V., Römer, G.R.B.E., Skolski, J.Z.P., Vincenc Obona, J., Visser, B.   +7 more
core   +1 more source

Dapagliflozin prevents methylglyoxal‐induced retinal cell death in ARPE‐19 cells

FEBS Open Bio, EarlyView.
Diabetic macular oedema is a diabetes complication of the eye, which may lead to permanent blindness. ARPE‐19 are human retinal cells used to study retinal diseases and potential therapeutics. Methylglyoxal is a compound increased in uncontrolled diabetes due to elevated blood glucose.
Naina Trivedi, Zainab Quraishi, Shima Khezri Azizi Far, Madaleine Ward, Aidan Conway, Reem Abdulredha, Eman Mshari, Yousif A. Shamsaldeen   +7 more
wiley   +1 more source

Análise invasiva e não invasiva do fuso meiótico de oócitos humanos obtidos de ciclos estimulados: dados preliminares Invasive and noninvasive analysis of the meiotic spindle of human oocytes obtained from stimulate cycles: preliminary results

Revista Brasileira de Ginecologia e Obstetrícia, 2012
OBJETIVOS: Avaliar a concordância entre as técnicas de microscopia de polarização e microscopia confocal na avaliação do fuso meiótico de oócitos humanos maturados in vivo.
Luciana Azôr Dib, Michele Gomes da Broi, Maria Cristina Picinato Medeiros de Araújo, Roberta Cristina Giorgenon, Rui Alberto Ferriani, Paula Andrea de Albuquerque Sales Navarro   +5 more
doaj   +1 more source

Comparative performance of airyscan and structured illumination superresolution microscopy in the study of the surface texture and 3D shape of pollen

, 2018
The visualization of taxonomically diagnostic features of individual pollen grains can be a challenge for many ecologically and phylogenetically important pollen types.
Allen, Andersen, Anderson, Chaturvedi, Dan, Faegri, Gustafsson, Gustafsson, Habuchi, Han, Heintzmann, Hesse, Holt, Huang, Jost, Kasuboski, Leung, Long, Lukosz, Mander, Mander, Mander, Muller, Nelson, Page, Peltre, Punt, Punyasena, Reimer, Sheppard, Sivaguru, Sivaguru, Sun, Tcheng, Traverse, Urban, Urban, Urban, Weiss   +38 more
core   +1 more source