Results 81 to 90 of about 2,341,220 (352)

MITF maintains genome stability in nonmelanocyte lineages

Molecular Oncology, EarlyView.
MITF is essential for melanocyte survival and acts as an oncogene in 10%–20% of melanomas. We show that MITF depletion causes genome instability in nonmelanocytic cells, leading to LATS2‐mediated P53 activation, cell cycle arrest, and apoptosis. This study highlights the role of MITF as a genome maintenance factor beyond the melanocyte lineage. Created
Drifa H. Gudmundsdottir, Adrián López García de Lomana, Thejus B. Venkatesh, Kritika Kirty, Snaevar Sigurdsson, Linda Vidarsdottir, Ramile Dilshat, Erla Sveinbjornsdottir, Snaedis Ragnarsdottir, Daniel H Magnusson, Maria R. Bustos, Eirikur Steingrimsson, Thorkell Gudjonsson, Stefan Sigurdsson   +13 more
wiley   +1 more source

Quantifying receptor trafficking and colocalization with confocal microscopy.

Methods, 2017
Confocal microscopy is a powerful tool for the study of cellular receptor trafficking and endocytosis. Unbiased and robust image analysis workflows are required for the identification, and study, of aberrant trafficking.
Jeremy A. Pike, I. Styles, J. Rappoport, J. Heath   +3 more
semanticscholar   +1 more source

Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization

Molecular Oncology, EarlyView.
In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu, Nicolas J. Niklaus, Anna M. Schläfli, Igor Tokarchuk, Magali Humbert, Federico La Manna, Bich Vu, David Maul, Ramin Radpour, Marianna Kruithof‐de Julio, Inti Zlobec, Jörn Dengjel, Bruce E. Torbett, Mario P. Tschan   +13 more
wiley   +1 more source

Analysis of corneal morphologic and pathologic changes in early-stage congenital aniridic keratopathy

International Journal of Ophthalmology, 2017
AIM: To determine typical corneal changes of congenital aniridic keratopathy (CAK) using corneal topography and confocal systems, and to identify characteristics that might assist in early diagnosis.
Juan Du, Rong-Qiang Liu, Lei Ye, Zhi-Hui Li, Feng-Tu Zhao, Nan Jiang, Lin-Hong Ye, Yi Shao   +7 more
doaj   +1 more source

Live tracking of moving samples in confocal microscopy for vertically grown roots

eLife, 2017
Roots navigate through soil integrating environmental signals to orient their growth. The Arabidopsis root is a widely used model for developmental, physiological and cell biological studies.
Daniel von Wangenheim, R. Hauschild, M. Fendrych, Vanessa Barone, E. Benková, J. Friml   +5 more
semanticscholar   +1 more source

Electron microscopic measurement of the size of the optical focus in laser scanning microscopy

, 2012
We describe a method for measuring the lateral focal spot size of a multiphoton laser scanning microscope (LSM) with unprecedented accuracy. A specimen consisting of an aluminum film deposited on a glass coverslip was brought into focus in a LSM and the ...
McConnell, Gail
core   +1 more source

Patient therapy outcome modeling in cancer organoids is improved by cancer‐associated fibroblasts and organoid assembly convolution

Molecular Oncology, EarlyView.
Patient‐derived organoids (PDOs) from pancreatic, colorectal, and gastric cancers were used to evaluate standard and experimental therapies. Incorporating cancer‐associated fibroblasts (CAFs) into organoid cultures improved patient therapy outcome prediction.
Marcin Grochowski, Liudmyla Dolinchuk, Michał Jerzak, Albert Gandurski, Tomasz Grochowski, Weronika Wojtyś, Maciej Zadrożny, Wojciech Kaźmierczak, Małgorzata Lenarcik, Marta Matejak‐Górska, Radosław Samsel, Tomasz Olesiński, Dawid Walerych   +12 more
wiley   +1 more source

Smartphone confocal microscopy for imaging cellular structures in human skin in vivo.

Biomedical Optics Express, 2018
We report development of a low-cost smartphone confocal microscope and its first demonstration of in vivo human skin imaging. The smartphone confocal microscope uses a slit aperture and diffraction grating to conduct two-dimensional confocal imaging ...
Esther, E., Freeman, A. Semeere, Hany A Osman, G. Peterson, M. Rajadhyaksha, S. González, Jeffery, N., Martin, R., R. Anderson, Guillermo, J., G J Tearney, Dongkyun Kang   +16 more
semanticscholar   +1 more source

Comparison of Confocal Microscopy and Scheimpflug Camera Findings in Avellino Corneal Dystrophy

, 2012
In this case report, a literature-guided comparative analysis of confocal microscopy and Scheimpflug camera findings of an adult with Avellino corneal dystrophy was performed.
Murat Küçükevcilioğlu, Fazıl Cüneyt Erdurman, Osman Melih Ceylan, Volkan Hürmeriç   +3 more
core   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source