Results 71 to 80 of about 3,090,289 (293)

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Probing the Atomic Structures of Synthetic Monolayer and Bilayer Hexagonal Boron Nitride Using Electron Microscopy

, 2016
Monolayer hexagonal boron nitride (h-BN) is a phenomenal two-dimensional material; most of its physical properties rival those of graphene because of their structural similarities.
Lin, Jinjun, McCulloch, Dougal G., Teo, Edwin Hang Tong, Tay, Roland Yingjie, Tsang, Siu Hon   +4 more
core   +1 more source

Structural basis for CRISPR RNA-guided DNA recognition by Cascade [PDF]

, 2011
The CRISPR (clustered regularly interspaced short palindromic repeats) immune system in prokaryotes uses small guide RNAs to neutralize invading viruses and plasmids.
Beijer, Marieke R., Mark J Dickman, Dickman, Mark J.,, Snijders, AP, Matthijs M Jore, Rolf Wagner, Sakharam P Waghmare, Beijer, Marieke, Wagner, Rolf, Brouns, Stan J. J., Barendregt, Arjan,, Snijders, Ambrosius, Dickman, Mark, Oost, J., van der, Arjan Barendregt, Boekema, Egbert, Dickman, Mark J., Bultema, J.B., Boekema, EJ, Waghmare, Sakharam P.,, Brouns, S.J.J., Wurm, Reinhild,, Westra, ER, Pul, U, Boekema, Egbert J., Doudna, Jennifer, Pul, Ümit,, Beijer, Marieke R.,, Egbert J Boekema, Jelle B Bultema, Westra, Edze, Snijders, Ambrosius P.L.,, Lundgren, Magnus, Jennifer A Doudna, Bultema, Jelle B.,, Wagner, R, John van der Oost, Westra, Edze R.,, van der Oost, J, Beijer, M.R., Wurm, R, Beijer, MR, Edze R Westra, Doudna, Jennifer A.,, Kaihong Zhou, Duijn, E., van, Jore, M.M., Heck, AJ, van der Oost, J., Waghmare, Sakharam, Jore, MM, Westra, E.R., Brouns, SJ, Doudna, Jennifer A., Wurm, Reinhild, Wagner, Rolf,, Ambrosius P L Snijders, Zhou, K, Barendregt, Arjan, Albert J R Heck, Brouns, Stan J.J.,, Magnus Lundgren, Pul, Uemit, Boekema, Egbert J.,, van Duijn, Esther, Snijders, Ambrosius P. L., Heck, Albert J. R., Lundgren, M, Pul, Ümit, Zhou, Kaihong,, Oost, John van der,, Blake Wiedenheft, Wiedenheft, Blake,, Wiedenheft, Blake, Waghmare, SP, van der Oost, John, Reinhild Wurm, Stan J J Brouns, Brouns, Stan, Jore, Matthijs M.,, Waghmare, Sakharam P., Bultema, Jelle, Zhou, Kaihong, Dickman, MJ, Lundgren, Magnus,, Barendregt, A, Bultema, JB, Jore, Matthijs, Duijn, Esther van,, Jore, Matthijs M., Lundgren, N.M.J., Ümit Pul, Marieke R Beijer, Wiedenheft, B, van Duijn, E., Heck, Albert J.R.,, Heck, Albert, Bultema, Jelle B., van Duijn, E, Westra, Edze R., Esther van Duijn, Doudna, JA   +101 more
core   +3 more sources

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Comparative 3D ultrastructure of Plasmodium falciparum gametocytes

Nature Communications
Despite the enormous significance of malaria parasites for global health, some basic features of their ultrastructure remain obscure. Here, we apply high-resolution volumetric electron microscopy to examine and compare the ultrastructure of the ...
Felix Evers, Rona Roverts, Cas Boshoven, Mariska Kea-te Lindert, Julie M. J. Verhoef, Nico Sommerdijk, Robert E. Sinden, Anat Akiva, Taco W. A. Kooij   +8 more
doaj   +1 more source

Electron Microscopy Characterization of Ni-Cr-B-Si-C Laser Deposited Coatings [PDF]

, 2013
During laser deposition of Ni-Cr-B-Si-C alloys with high amounts of Cr and B, various microstructures and phases can be generated from the same chemical composition that results in heterogeneous properties in the clad layer.
Ocelik, V., Rao, J.C.,, Ocelík, V.,, Hemmati, I., Hosson, J.Th.M. De,, Ocelik, V.; id_orcid, Hemmati, I.,, De Hosson, J. Th. M., Rao, J. C.   +8 more
core   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Anthelmintic Activity of Iron Oxide Nanoparticles Against the Goose Nematode Heterakis dispar (Schrank, 1790): An In Vitro Study

Veterinary Medicine International
In recent years, nanoparticles have been widely used in many fields, including biology and medicine. Helminthiasis pathogens continue to be detected in poultry farms, seriously affecting the quality of poultry meat.
Fuad Rzayev, Eldar Gasimov, Ali Nasirov, Sarvinaz Hajiyeva, Alakbar Huseynzada, Rustam Allahverdiyev, Milada Babayeva, Turana Huseynova, Sevinj Allahverdiyeva, Nigar Guliyeva, Sabina Israfilova, Mehri Seyidbeyli, Aysun Keskin, Gunay Rzayeva   +13 more
doaj   +1 more source

Scanning Electron Microscopy

Current Protocols
AbstractScanning electron microscopy (SEM) remains distinct in its ability to allow topographical visualization of structures. Key elements to consider for successful examination of biological specimens include appropriate preparative and imaging techniques.
Elizabeth R. Fischer, Bryan T. Hansen, Vinod Nair, Forrest H. Hoyt, Cindi L. Schwartz, David W. Dorward   +5 more
openaire   +2 more sources

A cross-sectional transmission electron microscopy study of iron recovered from a laser-heated diamond anvil cell [PDF]

, 2008
This paper discusses the use of a focused ion beam for the preparation of cross-sectional transmission electron microscopy specimens from small samples, typically 150 micron in diameter, that have been recovered from a laser-heated diamond anvil cell. We
Greaves, Graeme, Jephcoat, A P, Donnelly, S. E., Bouhifd, M A   +3 more
core   +1 more source