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Correlative Organelle Microscopy: Fluorescence Guided Volume Electron Microscopy of Intracellular Processes [PDF]
Frontiers in Cell and Developmental Biology, 2022 Intracellular processes depend on a strict spatial and temporal organization of proteins and organelles. Therefore, directly linking molecular to nanoscale ultrastructural information is crucial in understanding cellular physiology.
Sergey V. Loginov +9 more
doaj +3 more sources
A Poisson-Gaussian Denoising Dataset with Real Fluorescence Microscopy Images [PDF]
arXiv, 2018 Fluorescence microscopy has enabled a dramatic development in modern biology. Due to its inherently weak signal, fluorescence microscopy is not only much noisier than photography, but also presented with Poisson-Gaussian noise where Poisson noise, or shot noise, is the dominating noise source.
Yide Zhang +6 more
arxiv +3 more sources
Three-dimensional virtual refocusing of fluorescence microscopy images using deep learning [PDF]
Nature Methods (2019), 2019 Three-dimensional (3D) fluorescence microscopy in general requires axial scanning to capture images of a sample at different planes. Here we demonstrate that a deep convolutional neural network can be trained to virtually refocus a 2D fluorescence image onto user-defined 3D surfaces within the sample volume.
Yichen Wu +7 more
arxiv +3 more sources
Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations
Cells, 2021 Fluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular level. Micrographs from fixed and live-cell imaging procedures feature in a plethora of scientific articles for the field of cell ...
Shane M. Hickey +12 more
doaj +2 more sources
Advanced Methods in Fluorescence Microscopy [PDF]
Analytical Cellular Pathology, 2013 It requires a good deal of will power to resist hyperbole in considering the advances that have been achieved in fluorescence microscopy in the last 25 years.
Luke Fritzky, David Lagunoff
doaj +5 more sources
Plasmonics Enhanced Smartphone Fluorescence Microscopy
Scientific Reports, 2017 Smartphone fluorescence microscopy has various applications in point-of-care (POC) testing and diagnostics, ranging from e.g., quantification of immunoassays, detection of microorganisms, to sensing of viruses.
Qingshan Wei +9 more
doaj +2 more sources
Cold Spring Harbor Protocols, 2014 Fluorescence microscopy is a major tool with which to monitor cell physiology. Although the concepts of fluorescence and its optical separation using filters remain similar, microscope design varies with the aim of increasing image contrast and spatial resolution.
Sanderson, Michael J +3 more
openaire +7 more sources
Ångström-resolution fluorescence microscopy
Nature, 2023 The authors introduce a single-molecule DNA-barcoding method, resolution enhancement by sequential imaging, that improves the resolution of fluorescence microscopy down to the Ångström scale using off-the-shelf fluorescence microscopy hardware and ...
Susanne C. M. Reinhardt +11 more
semanticscholar +1 more source
Linear Combination Properties of the Phasor Space in Fluorescence Imaging
Sensors, 2022 The phasor approach to fluorescence lifetime imaging, and more recently hyperspectral fluorescence imaging, has increased the use of these techniques, and improved the ease and intuitiveness of the data analysis.
Belén Torrado +2 more
doaj +1 more source
Total Internal Reflection Fluorescence (TIRF) Microscopy
Current Protocols, 2022 Total internal reflection fluorescence (TIRF) microscopy (TIRFM) is an elegant optical technique that provides for the excitation of fluorophores in an extremely thin axial region (“optical section”).
K. Fish
semanticscholar +1 more source