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Correlative Organelle Microscopy: Fluorescence Guided Volume Electron Microscopy of Intracellular Processes [PDF]

open access: yesFrontiers in Cell and Developmental Biology, 2022
Intracellular processes depend on a strict spatial and temporal organization of proteins and organelles. Therefore, directly linking molecular to nanoscale ultrastructural information is crucial in understanding cellular physiology.
Sergey V. Loginov   +9 more
doaj   +3 more sources

Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations

open access: yesCells, 2021
Fluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular level. Micrographs from fixed and live-cell imaging procedures feature in a plethora of scientific articles for the field of cell ...
Shane M. Hickey   +12 more
doaj   +2 more sources

Plasmonics Enhanced Smartphone Fluorescence Microscopy

open access: yesScientific Reports, 2017
Smartphone fluorescence microscopy has various applications in point-of-care (POC) testing and diagnostics, ranging from e.g., quantification of immunoassays, detection of microorganisms, to sensing of viruses.
Qingshan Wei   +9 more
doaj   +2 more sources

Fluorescence Microscopy [PDF]

open access: yesCold Spring Harbor Protocols, 2014
Fluorescence microscopy is a major tool with which to monitor cell physiology. Although the concepts of fluorescence and its optical separation using filters remain similar, microscope design varies with the aim of increasing image contrast and spatial resolution.
Sanderson, Michael J   +3 more
  +8 more sources

Linear Combination Properties of the Phasor Space in Fluorescence Imaging

open access: yesSensors, 2022
The phasor approach to fluorescence lifetime imaging, and more recently hyperspectral fluorescence imaging, has increased the use of these techniques, and improved the ease and intuitiveness of the data analysis.
Belén Torrado   +2 more
doaj   +1 more source

Ångström-resolution fluorescence microscopy

open access: yesNature, 2023
The authors introduce a single-molecule DNA-barcoding method, resolution enhancement by sequential imaging, that improves the resolution of fluorescence microscopy down to the Ångström scale using off-the-shelf fluorescence microscopy hardware and ...
Susanne C. M. Reinhardt   +11 more
semanticscholar   +1 more source

Fluorescence-Detected Mid-Infrared Photothermal Microscopy. [PDF]

open access: yesJournal of the American Chemical Society, 2021
We demonstrate instrumentation and methods to enable fluorescence-detected photothermal infrared (F-PTIR) microscopy and then demonstrate the utility of F-PTIR to characterize the composition within phase-separated domains of model amorphous solid ...
Minghe Li   +9 more
semanticscholar   +1 more source

Intravital fluorescence microscopy with negative contrast.

open access: yesPLoS ONE, 2021
Advances in intravital microscopy (IVM) have enabled the studies of cellular organization and dynamics in the native microenvironment of intact organisms with minimal perturbation.
Juwell W Wu   +9 more
doaj   +2 more sources

Super-resolution fluorescence polarization microscopy [PDF]

open access: yesJournal of Innovative Optical Health Sciences, 2018
Fluorescence polarization is related to the dipole orientation of chromophores, making fluorescence polarization microscopy possible to reveal structures and functions of tagged cellular organelles and biological macromolecules.
Karl Zhanghao   +4 more
doaj   +1 more source

Low-Light Photodetectors for Fluorescence Microscopy

open access: yesApplied Sciences, 2021
Over the years, fluorescence microscopy has evolved and has become a necessary element of life science studies. Microscopy has elucidated biological processes in live cells and organisms, and also enabled tracking of biomolecules in real time ...
Hiroaki Yokota   +3 more
doaj   +1 more source

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