3D volume rendering of invertebrates using light-sheet fluorescence microscopy [PDF]
, 2015 Light-Sheet Fluorescence Microscopy (LSFM) has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms with low photo-damage and fast acquisition rates.
Gualda Manzano, Emilio José +1 more
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, 2013 Optical microscopy has developed as an indispensable tool for Arabidopsis cell biology. This is due to the high sensitivity, good spatial resolution, minimal invasiveness, and availability of autofluorescent proteins, which can be specifically fused to a distinct protein of interest.
Sébastien, Peter +2 more
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Light Sheet Fluorescence Microscopy Illuminating Soft Matter
Frontiers in Physics, 2021 Volumetric microscopic imaging data acquired at high speeds is often needed in studies of soft matter. Several microscopy techniques exist for this purpose, but a relative newcomer is light sheet fluorescence microscopy (LSFM). This microscopy method has
Ruilin You, Ryan McGorty
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Super-Resolution Microscopy: A Virus’ Eye View of the Cell [PDF]
, 2014 It is difficult to observe the molecular choreography between viruses and host cell components, as they exist on a spatial scale beyond the reach of conventional microscopy.
Grove, J, Grove, Joe, Joe Grove
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Correlative stochastic optical reconstruction microscopy and electron microscopy. [PDF]
PLoS ONE, 2015 Correlative fluorescence light microscopy and electron microscopy allows the imaging of spatial distributions of specific biomolecules in the context of cellular ultrastructure.
Doory Kim +5 more
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Quantitative Live-Cell Imaging of Human Immunodeficiency Virus (HIV-1) Assembly [PDF]
, 2012 Advances in fluorescence methodologies make it possible to investigate biological systems in unprecedented detail. Over the last few years, quantitative live-cell imaging has increasingly been used to study the dynamic interactions of viruses with cells ...
Barbara Müller +5 more
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Comparison of the new OPS Imaging technique with intravital microscopy: Analysis of the colon microcirculation [PDF]
, 2002 Background. The OPS imaging technique has been introduced for in vivo assessment of microcirculation in humans. The aim of this study was to validate the new technique against intravital fluorescence microscopy (IFM) for the visualization of colon ...
Langer, S., Biberthaler, Peter
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Developing Digital Photomicroscopy
Cells, 2022 (1) The need for efficient ways of recording and presenting multicolour immunohistochemistry images in a pioneering laboratory developing new techniques motivated a move away from photography to electronic and ultimately digital photomicroscopy.
Kingsley Micklem
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Wavefront shaping of a Bessel light field enhances light sheet microscopy with scattered light
, 2014 The project was supported by the UK Engineering and Physical Sciences Research Council, RS MacDonald Charitable Trust, SULSA, and the St. Andrews 600th anniversary BRAINS appeal. K. D.
Vettenburg, T.; id_orcid +11 more
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Applying two-photon excitation fluorescence lifetime imaging microscopy to study photosynthesis in plant leaves [PDF]
, 2009 This study investigates to which extent two-photon excitation (TPE) fluorescence lifetime imaging microscopy can be applied to study picosecond fluorescence kinetics of individual chloroplasts in leaves.
van Amerongen, H. +6 more
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