Results 51 to 60 of about 768,563 (305)

Epi-Fluorescence Microscopy [PDF]

, 2012
Epi-fluorescence microscopy is available in most life sciences research laboratories, and when optimized can be a central laboratory tool. In this chapter, the epi-fluorescence light path is introduced and the various components are discussed in detail.
Donna J, Webb, Claire M, Brown
openaire   +2 more sources

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Calibrating Fluorescence Microscopy With 3D-Speckler (3D Fluorescence Speckle Analyzer)

Bio-Protocol
Fluorescence microscopy has been widely accessible and indispensable in cell biology research. This technique enables researchers to label targets, ranging from individual entities to multiple groups, with fluorescent markers.
Chieh-Chang Lin, Aussie Suzuki
doaj   +1 more source

Viral particle imaging by super-resolution fluorescence microscopy

Chemical Physics Impact, 2021
Fluorescence microscopy provides a facile imaging methodology and potentials for imaging viruses in vivo. However, it is limited by the diffraction limit of light microscopy which is above the size of the virus particles.
Stefania Castelletto, Alberto Boretti
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Wide-Field Multi-Parameter FLIM: Long-Term Minimal Invasive Observation of Proteins in Living Cells.

, 2011
Time-domain Fluorescence Lifetime Imaging Microscopy (FLIM) is a remarkable tool to monitor the dynamics of fluorophore-tagged protein domains inside living cells.
Yury Prokazov, Turbin, E., Juan Llopis, Duci, A., Roland Hartig, Götze, C., Zuschratter, W., Christian Götze, Prokazov, Y., Hartig, R., Evgeny Turbin, Antonie J. W. G. Visser, Marco Vitali, Visser, A.J.W.G., Alessandro Duci, Werner Zuschratter, Vitali, M., Fernando Picazo, Picazo, F., Llopis, J., Antonie J W G Visser   +20 more
core   +1 more source

A Technical Note on Quantum Dots for Multi-Color Fluorescence in situ Hybridization [PDF]

, 2009
Quantum dots (Qdots) are semiconductor nanocrystals, which are photo-stable, show bright fluorescence with narrow, symmetric emission spectra and are available in multiple resolvable colors.
Müller, Stefan, Cremer, T., Neusser, M., Grasser, F., Cremer, M.   +4 more
core   +1 more source

Fast image reconstruction for fluorescence microscopy

AIP Advances, 2012
Real-time image reconstruction is essential for improving the temporal resolution of fluorescence microscopy. A number of unavoidable processes such as, optical aberration, noise and scattering degrade image quality, thereby making image reconstruction ...
Mahesh Ravi Varma, K. Rajan, Partha Pratim Mondal   +2 more
doaj   +1 more source

Fluorescence radial fluctuation enables two-photon super-resolution microscopy

Frontiers in Cellular Neuroscience, 2023
Despite recent improvements in microscopy, it is still difficult to apply super-resolution microscopy for deep imaging due to the deterioration of light convergence properties in thick specimens.
Motosuke Tsutsumi, Motosuke Tsutsumi, Taiga Takahashi, Taiga Takahashi, Kentaro Kobayashi, Tomomi Nemoto, Tomomi Nemoto, Tomomi Nemoto   +7 more
doaj   +1 more source

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

FEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga, Toshiki Takahashi, Akiko Kuma, Hiroyuki Kawahara   +3 more
wiley   +1 more source