Results 61 to 70 of about 1,583,891 (182)

Structural Changes of Yellow Cameleon Domains Observed by Quantitative FRET Analysis and Polarized Fluorescence Correlation Spectroscopy [PDF]

, 2008
Förster resonance energy transfer (FRET) is a widely used method for monitoring interactions between or within biological macromolecules conjugated with suitable donor-acceptor pairs.
Laptenok, S., Visser, N.V., Kalinin, S., Hornen, H., Hoek, A., van, Kühnemuth, R., Borst, J.W., van Hoek, A., Westphal, A.H., Aker, J., Aker, J.C.M., Kyhnemuth, R., Laptenok, S.P., Seidel, C.A.M., Visser, A.J.W.G.   +14 more
core   +1 more source

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Real-Time analysis and visualization for single-molecule based super-resolution microscopy [PDF]

, 2013
Accurate multidimensional localization of isolated fluorescent emitters is a time consuming process in single-molecule based super-resolution microscopy.
Jean-Baptiste Sibarita, Kechkar, Adel, Choquet, Daniel, Deepak Nair, Mike Heilemann, Nair, Deepak, Daniel Choquet, Sibarita, Jean-Baptiste, Adel Kechkar, Heilemann, Mike   +9 more
core   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Laser-based fluorescence microscopy

, 2002
[Introduction]:Many microbiologists will be familiar with transmission fluorescence microscopy as well the improved epifluorescence microscopy. However, microbiologists are generally less familiar with confocal laser scanning microscopy (CLSM), despite ...
Learmonth, Robert P.
core   +1 more source

Applying two-photon excitation fluorescence lifetime imaging microscopy to study photosynthesis in plant leaves [PDF]

, 2009
This study investigates to which extent two-photon excitation (TPE) fluorescence lifetime imaging microscopy can be applied to study picosecond fluorescence kinetics of individual chloroplasts in leaves.
van Amerongen, H., Koen Broess, Amerongen, H., van, Jan Willem Borst, Borst, J.W., Broess, K., Herbert van Amerongen   +6 more
core   +1 more source

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Extracting fluorescent reporter time courses of cell lineages from high-throughput microscopy at low temporal resolution [PDF]

, 2011
The extraction of fluorescence time course data is a major bottleneck in high-throughput live-cell microscopy. Here we present an extendible framework based on the open-source image analysis software ImageJ, which aims in particular at analyzing the ...
Ott Sascha, Georgy Koentges, Mike J Downey, Jeziorska, DM, Tamai T. Katherine, Downey, Mike J., Downey Mike J., T. Katherine Tamai, Keith W, Bretschneider, T, Tamai, T Katherine, Downey, Mike J, Vance, KW, Ott, S, Mike J. Downey, Koentges, Georgy, Downey, MJ, Keith W Vance, Danuta M. Jeziorska, Bretschneider Till, Vance, Keith W., Jeziorska Danuta M., Till Bretschneider, Danuta M Jeziorska, Koentges Georgy, Sascha Ott, Vance Keith W., Koentges, G, T Katherine Tamai, Jeziorska, Danuta M, Bretschneider, Till, Jeziorska, Danuta M., Vance, Keith W, Ott, Sascha, Tamai, T. Katherine, Tamai, TK   +35 more
core   +1 more source