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Multiphoton Fluorescence Microscopy [PDF]
Methods, 2001 Multiphoton fluorescence microscopy has now become a relatively common tool among biophysicists and biologists. The intrinsic sectioning achievable by multiphoton excitation provides a simple means to excite a small volume inside cells and tissues.
Gratton, Enrico +3 more
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Frontiers in Immunology, 2015 Numerous studies emphasize the relevance of thymocyte-thymic epithelial cell (TECs) interactions for the functional maturation of intrathymic T lymphocytes.
Javier eGarcia-Ceca +6 more
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Bleaching‐Resistant Super‐Resolution Fluorescence Microscopy
Advanced Science, 2022 Photobleaching is the permanent loss of fluorescence after extended exposure to light and is a major limiting factor in super‐resolution microscopy (SRM) that restricts spatiotemporal resolution and observation time.
Jiwoong Kwon +2 more
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Deep-learning-based methods for super-resolution fluorescence microscopy
Journal of Innovative Optical Health Sciences, 2023 The algorithm used for reconstruction or resolution enhancement is one of the factors affecting the quality of super-resolution images obtained by fluorescence microscopy. Deep-learning-based algorithms have achieved state-of-the-art performance in super-
Jianhui Liao +3 more
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Frontiers in Endocrinology, 2018 For a long time, the effects of distinct Eph tyrosine kinase receptors and their ligands, ephrins on the structure, immunophenotype, and development of thymus and their main cell components, thymocytes (T) and thymic epithelial cells (TECs), have been ...
Juan José Muñoz +6 more
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Fluorescence Microscopy in Adeno-Associated Virus Research
Viruses, 2023 Research on adeno-associated virus (AAV) and its recombinant vectors as well as on fluorescence microscopy imaging is rapidly progressing driven by clinical applications and new technologies, respectively.
Susanne K. Golm +2 more
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A statistical resolution measure of fluorescence microscopy with finite photons [PDF]
, 2023 First discovered by Ernest Abbe in 1873, the resolution limit of a far-field microscope is considered determined by the numerical aperture and wavelength of light, approximately $\lambda$/2NA. With the advent of modern fluorescence microscopy and nanoscopy methods over the last century, it is recognized that Abbe's resolution definition alone could not
arxiv +1 more source
Multiple airy beams light-sheet fluorescence microscopy
Frontiers in Physics, 2022 Light-sheet fluorescence microscopy (LSFM) is a kind of volumetric imaging methodology suited for long term living specimens at high temporal-spatial resolution.
Shuangyu Gu +9 more
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Beyond Range: Innovating Fluorescence Microscopy
Remote Sensing, 2012 Time-of-Flight (ToF) technologies are developed mainly for range estimations in industrial applications or consumer products. Recently, it was realized that ToF sensors could also be used for the detection of fluorescence and of the minute changes in the
Alessandro Esposito
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Scanless multitarget-matching multiphoton excitation fluorescence microscopy [PDF]
Journal of Innovative Optical Health Sciences, 2018 Using the combination of a reflective blazed grating and a reflective phase-only diffractive spatial light modulator (SLM), scanless multitarget-matching multiphoton excitation fluorescence microscopy (SMTM-MPM) was achieved.
Junpeng Qiu +4 more
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