Results 31 to 40 of about 625,799 (289)
Fluorescence antibunching microscopy [PDF]
, 2011 Breaking the diffraction limit in microscopy by utilizing quantum properties of light has been the goal of intense research in the recent years. We propose a quantum superresolution technique based on non-classical emission statistics of fluorescent markers, routinely used as contrast labels for bio-imaging.
arxiv +1 more source
Simultaneous off-axis multiplexed holography and regular fluorescence microscopy of biological cells [PDF]
Opt. Lett. 43, 2587-2590 (2018), 2019 We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera. Our system is based on an interferometric multiplexing module, externally positioned at the exit of an optical microscope.
arxiv +1 more source
Bridging fluorescence microscopy and electron microscopy [PDF]
Histochemistry and Cell Biology, 2008 Development of new fluorescent probes and fluorescence microscopes has led to new ways to study cell biology. With the emergence of specialized microscopy units at most universities and research centers, the use of these techniques is well within reach for a broad research community.
openaire +4 more sources
Compressed sensing in fluorescence microscopy
Progress in Biophysics and Molecular Biology, 2022 Compressed sensing (CS) is a signal processing approach that solves ill-posed inverse problems, from under-sampled data with respect to the Nyquist criterium. CS exploits sparsity constraints based on the knowledge of prior information, relative to the structure of the object in the spatial or other domains.
Calisesi G. +6 more
openaire +6 more sources
Cells, 2021 Fluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular level. Micrographs from fixed and live-cell imaging procedures feature in a plethora of scientific articles for the field of cell ...
Shane M. Hickey +12 more
doaj +1 more source
Fast image reconstruction for fluorescence microscopy
AIP Advances, 2012 Real-time image reconstruction is essential for improving the temporal resolution of fluorescence microscopy. A number of unavoidable processes such as, optical aberration, noise and scattering degrade image quality, thereby making image reconstruction ...
Mahesh Ravi Varma +2 more
doaj +1 more source
Fourier imaging study of efficient near-field optical coupling in solid immersion fluorescence microscopy [PDF]
, 2002 We measured images and Fourier images of fluorescence for 0.11- and 0.22-$\mu$m-diameter dye-doped polystyrene micro-sphere beads on a solid immersion lens, and experimentally verified strongly-angle-dependent fluorescence intensities due to efficient near-field optical coupling in solid immersion fluorescence microscopy. The results are interpreted in
arxiv +1 more source
Probing subtle fluorescence dynamics in cellular proteins by streak camera based Fluorescence Lifetime Imaging Microscopy [PDF]
, 2003 We report the cell biological applications of a recently developed multiphoton fluorescence lifetime imaging microscopy system using a streak camera (StreakFLIM). The system was calibrated with standard fluorophore specimens and was shown to have high accuracy and reproducibility.
arxiv +1 more source
ixFLIM: Interferometric Excitation Fluorescence Lifetime Imaging Microscopy [PDF]
arXiv, 2023 Fluorescence lifetime imaging microscopy (FLIM) is a well-established technique with numerous imaging applications. Yet, one of the limitations of FLIM is that it provides information about the emitting state only. Here, we present an extension of FLIM by interferometric measurement of fluorescence excitation spectra.
arxiv
Integral refractive index imaging of flowing cell nuclei using quantitative phase microscopy combined with fluorescence microscopy [PDF]
Opt. Express 9, 1177-1189 (2018), 2019 We suggest a new multimodal imaging technique for quantitatively measuring the integral (thickness-average) refractive index of the nuclei of live biological cells in suspension. For this aim, we combined quantitative phase microscopy with simultaneous 2-D fluorescence microscopy.
arxiv +1 more source