Results 61 to 70 of about 3,074,988 (359)
ixFLIM: Interferometric Excitation Fluorescence Lifetime Imaging Microscopy [PDF]
arXiv, 2023 Fluorescence lifetime imaging microscopy (FLIM) is a well-established technique with numerous imaging applications. Yet, one of the limitations of FLIM is that it provides information about the emitting state only. Here, we present an extension of FLIM by interferometric measurement of fluorescence excitation spectra.
arxiv
Integral refractive index imaging of flowing cell nuclei using quantitative phase microscopy combined with fluorescence microscopy [PDF]
Opt. Express 9, 1177-1189 (2018), 2019 We suggest a new multimodal imaging technique for quantitatively measuring the integral (thickness-average) refractive index of the nuclei of live biological cells in suspension. For this aim, we combined quantitative phase microscopy with simultaneous 2-D fluorescence microscopy.
arxiv +1 more source
Microscopy and Microanalysis, 2019 Correlated light and electron microscopy (CLEM) has become a popular technique for combining the protein-specific labeling of fluorescence with electron microscopy, both at room and cryogenic temperatures.
L. Metskas, John A. G. Briggs
semanticscholar +1 more source
Making tau amyloid models in vitro: a crucial and underestimated challenge
FEBS Letters, EarlyView.This review highlights the challenges of producing in vitro amyloid assemblies of the tau protein. We review how accurately the existing protocols mimic tau deposits found in the brain of patients affected with tauopathies. We discuss the important properties that should be considered when forming amyloids and the benchmarks that should be used to ...
Julien Broc, Clara Piersson, Yann Fichou
wiley +1 more source
miR-203 drives breast cancer cell differentiation
Breast Cancer Research, 2023 A hallmark of many malignant tumors is dedifferentiated (immature) cells bearing slight or no resemblance to the normal cells from which the cancer originated.
Nuria G. Martínez-Illescas +20 more
doaj +1 more source
Detection of fluorescence in situ hybridization on human metaphase chromosomes by near-field scanning optical microscopy [PDF]
, 1995 Fluorescence in situ hybridization signals on human metaphase chromosomes are detected by a near-field scanning optical microscope. This makes it possible to localize and identify several fluorescently labeled genomic DNA fragments on a single ...
Jalocha, A. +5 more
core +3 more sources
Identification of novel small molecule inhibitors of ETS transcription factors
FEBS Letters, EarlyView.ETS transcription factors play an essential role in tumourigenesis and are indispensable for sprouting angiogenesis, a hallmark of cancer, which fuels tumour expansion and dissemination. Thus, targeting ETS transcription factor function could represent an effective, multifaceted strategy to block tumour growth. The evolutionarily conserved E‐Twenty‐Six
Shaima Abdalla +9 more
wiley +1 more source
Three-dimensional bi-functional refractive index and fluorescence microscopy (BRIEF) [PDF]
arXiv, 2020 Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical property that quantitatively relates to cell morphology, mass, and stiffness.
arxiv
Common-path multimodal optical microscopy [PDF]
, 2009 We have developed a common-path multimodal optical microscopy system that is capable of using a single optical source and a single camera to image amplitude, phase, and fluorescence features of a biological specimen.
Kothapalli, Sri-Rajasekhar +4 more
core +1 more source
High performance, LED powered, waveguide based total internal reflection microscopy. [PDF]
, 2013 Total internal reflection fluorescence (TIRF) microscopy is a rapidly expanding optical technique with excellent surface sensitivity and limited background fluorescence. Commercially available TIRF systems are either objective based that employ expensive
Cohen, Daniel A +3 more
core +1 more source